Materatski Patrick, Varanda Carla, Carvalho Teresa, Dias António Bento, Campos M Doroteia, Rei Fernando, Félix Maria do Rosário
ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Instituto de Investigação e Formação Avançada, Universidade de Évora, Polo da Mitra, Ap. 94, 7006-554 Évora, Portugal.
ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Instituto de Investigação e Formação Avançada, Universidade de Évora, Polo da Mitra, Ap. 94, 7006-554 Évora, Portugal.
Fungal Biol. 2019 Jan;123(1):66-76. doi: 10.1016/j.funbio.2018.11.004. Epub 2018 Nov 22.
Fungal endophytes are micro-organisms that colonize healthy plant tissues without causing disease symptoms. They are described as plant growth and disease resistance promoters and have shown antimicrobial activity. The spatial-temporal distribution of endophytic communities in olive cultivars has been poorly explored. This study aims to investigate the richness and diversity of endophytic fungi in different seasons and sites, within the Alentejo region, Portugal. Additionally, and because the impact of some pathogenic fungi (e.g. Colletotrichum spp.) varies according to olive cultivars; three cultivars, Galega vulgar, Cobrançosa and Azeiteira, were sampled. 1868 fungal isolates were identified as belonging to 26 OTUs; 13 OTUs were identified to the genera level and 13 to species level. Cultivar Galega vulgar and season autumn showed significant higher values in terms of endophytic richness and diversity. At site level, Elvas showed the lowest fungal richness and diversity of fungal endophytes. This study reinforces the importance of exploring the combined spatio-temporal distribution of the endophytic biodiversity in different olive cultivars. Knowledge about endophytic communities may help to better understand their functions in plants hosts, such as their ecological dynamics with pathogenic fungi, which can be explored for their use as biocontrol agents.
真菌内生菌是定殖于健康植物组织而不引起病害症状的微生物。它们被描述为植物生长和抗病性促进剂，并已显示出抗菌活性。橄榄品种内生菌群落的时空分布研究较少。本研究旨在调查葡萄牙阿连特茹地区不同季节和地点内生真菌的丰富度和多样性。此外，由于一些致病真菌（如炭疽菌属）的影响因橄榄品种而异，因此对Galega vulgar、Cobrançosa和Azeiteira这三个品种进行了采样。1868株真菌分离物被鉴定为属于26个操作分类单元（OTU）；其中13个OTU被鉴定到属水平，13个被鉴定到种水平。Galega vulgar品种和秋季的内生菌丰富度和多样性显著更高。在地点水平上，埃尔瓦什的内生真菌丰富度和多样性最低。本研究强调了探索不同橄榄品种内生生物多样性时空分布组合的重要性。关于内生菌群落的知识可能有助于更好地理解它们在植物宿主中的功能，例如它们与致病真菌的生态动态，这可以用于探索将其用作生物防治剂。
