Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangkok Thailand.
Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangkok Thailand.
J Hazard Mater. 2021 Jun 5;411:125104. doi: 10.1016/j.jhazmat.2021.125104. Epub 2021 Jan 12.
Laccase is an effective biocatalyst in bioremediation process; however, the application of the enzyme is limited due to its cost, recovery, and stability. In this study, we developed, characterized and evaluated the efficiency of immobilized laccase on zinc oxide nanostructure to catalyze biodegradation of TBA in comparison to the suspended enzyme. The results showed that both immobilized and suspended laccase were capable of catalyzing TBA biodegradation; however, the efficiency of the immobilized laccase on TBA removal was higher than that of the suspended enzyme. The repeatability testing revealed the potential of the immobilized laccase for repeatedly catalyzing TBA biodegradation with storage capacity. While the V of immobilized enzyme was higher than suspended laccase (2.25 ± 0.542 mg TBA/h∙U vs. 1.47 ± 0.185 mg TBA/h∙U), the k of the immobilized enzyme was higher than the suspended laccase (67.9 ± 20.5 mg TBA/L vs. 33.5 ± 7.10 mg TBA/L). This suggests that the immobilized laccase is better in TBA removal, but has lower affinity with TBA than the suspended enzyme. Thus, immobilization of the enzyme can be applied to increase the efficiency and minimize the use of laccase for TBA remediation.
漆酶是生物修复过程中的一种有效生物催化剂；然而，由于其成本、回收和稳定性，该酶的应用受到限制。在这项研究中，我们开发、表征和评估了固定化漆酶在氧化锌纳米结构上催化 TBA 生物降解的效率，与悬浮酶相比。结果表明，固定化和悬浮漆酶都能够催化 TBA 生物降解；然而，固定化漆酶对 TBA 去除的效率高于悬浮酶。重复测试显示了固定化漆酶具有重复催化 TBA 生物降解和存储能力的潜力。虽然固定化酶的 V 值高于悬浮漆酶（2.25±0.542mg TBA/h·U 对 1.47±0.185mg TBA/h·U），但固定化酶的 k 值高于悬浮漆酶（67.9±20.5mg TBA/L 对 33.5±7.10mg TBA/L）。这表明固定化酶在 TBA 去除方面效果更好，但与悬浮酶相比，对 TBA 的亲和力较低。因此，酶的固定化可用于提高效率并最小化 TBA 修复中漆酶的使用。
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