Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Haridwar Road, Dehradun, 248005, Uttarakhand, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Environ Sci Pollut Res Int. 2023 Aug;30(36):84898-84917. doi: 10.1007/s11356-023-28275-5. Epub 2023 Jun 28.
Environmental concerns due to the release of industrial wastewater contaminated with dyes are becoming more and more intense with the increasing industrialization. Decolorization of industrial effluents has become the top priority due to the continuous demand for color-free discharge into the receiving water bodies. Different dye removal techniques have been developed, among which biodegradation by laccase enzyme is competitive. Laccase, as a green catalyst, has a high catalytic activity, generates less toxic by-products, and has been extensively researched in the field of remediation of dyes. However, laccase's significant catalytic activity could only be achieved after an effective immobilization step. Immobilization helps strengthen and stabilize the protein structure of laccase, thus enhancing its functional properties. Additionally, the reusability of immobilized laccase makes it an attractive alternative to traditional dye degradation technologies and in the realistic applications of water treatment, compared with free laccase. This review has elucidated different methods and the carriers used to immobilize laccase. Furthermore, the role of immobilized laccase in dye remediation and the prospects have been discussed.
由于工业废水的排放而导致的环境污染问题日益严重，这些工业废水中含有染料。为了满足将无色废水排入受纳水体的持续需求，工业废水的脱色已成为当务之急。已经开发了不同的染料去除技术，其中酶法生物降解具有竞争力。漆酶作为一种绿色催化剂，具有较高的催化活性，产生的有毒副产物较少，在染料修复领域得到了广泛的研究。然而，漆酶只有经过有效的固定化步骤才能发挥显著的催化活性。固定化有助于加强和稳定漆酶的蛋白质结构，从而提高其功能特性。此外，与游离漆酶相比，固定化漆酶的可重复使用性使其成为传统染料降解技术在水处理实际应用中的一种有吸引力的替代方法。本文综述了不同的方法和载体用于固定化漆酶。此外，还讨论了固定化漆酶在染料修复中的作用和前景。
