氢气通过调节长链非编码RNA MALAT1/miR-124-3p/EZH2轴抑制胃癌细胞的增殖和迁移。

Hydrogen inhibits the proliferation and migration of gastric cancer cells by modulating lncRNA MALAT1/miR-124-3p/EZH2 axis.

作者信息

Zhu Baocheng, Cui Hengguan, Xu Weiqiang

机构信息

Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.

Qingpu Branch of Zhongshan Hospital, Fudan University, 1158 Park East Road, Qingpu District, Shanghai, 201700, China.

出版信息

Cancer Cell Int. 2021 Jan 22;21(1):70. doi: 10.1186/s12935-020-01743-5.

DOI:10.1186/s12935-020-01743-5

PMID:33482814

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7821405/

Abstract

BACKGROUND

Gastric cancer is one of the most prevalent and deadly malignancies without efficient treatment option. This study aimed to investigate the effect of hydrogen gas on the behavior of gastric cancer cells.

METHODS

Gastric cancer cell lines MGC-803 and BGC-823 were treated with or without H /O gas mixture (66.7%:33.3% v/v). Proliferation and migration were assessed by MTT and scratch wound healing assays respectively. The expression of lncRNA MALAT1, miR-124-3p, and EZH2 was analyzed by real-time quantitative PCR and/or western blot. Tumor growth was estimated using xenograft mouse model.

RESULTS

H gas significantly inhibited gastric tumor growth in vivo and the proliferation, migration, and lncRNA MALAT1 and EZH2 expression of gastric cancer cells while upregulated miR-124-3p expression. LncRNA MALAT1 overexpression abolished all the aforementioned effects of H. LncRNA MALAT1 and miR-124-3p reciprocally inhibited the expression of each other. MiR-124-3p mimics abrogated lncRNA MALAT1 promoted EZH2 expression and gastric cancer cell proliferation and migration.

CONCLUSIONS

These data demonstrated that H might be developed as a therapeutics of gastric cancer and lncRNA MALAT1/miR-124-3p/EZH2 axis could be a target for intervention.

摘要

背景

胃癌是最常见且致命的恶性肿瘤之一，目前尚无有效的治疗方法。本研究旨在探讨氢气对胃癌细胞行为的影响。

方法

胃癌细胞系MGC - 803和BGC - 823分别用或不用H₂/O₂气体混合物（66.7%:33.3% v/v）处理。分别通过MTT法和划痕伤口愈合试验评估细胞增殖和迁移情况。通过实时定量PCR和/或蛋白质印迹分析lncRNA MALAT1、miR - 124 - 3p和EZH2的表达。使用异种移植小鼠模型评估肿瘤生长情况。

结果

氢气显著抑制体内胃癌肿瘤生长以及胃癌细胞的增殖、迁移、lncRNA MALAT1和EZH2表达，同时上调miR - 124 - 3p表达。lncRNA MALAT1过表达消除了氢气的上述所有作用。lncRNA MALAT1和miR - 124 - 3p相互抑制对方的表达。miR - 124 - 3p模拟物消除了lncRNA MALAT1促进EZH2表达以及胃癌细胞增殖和迁移的作用。