在中国云南省南部检测到伊蚊属昆虫中的蓝舌病毒。

Detection of bluetongue virus in Culicoides spp. in southern Yunnan Province, China.

机构信息

Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.

Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, NT, Australia.

出版信息

Parasit Vectors. 2021 Jan 22;14(1):68. doi: 10.1186/s13071-020-04518-z.

DOI:10.1186/s13071-020-04518-z

PMID:33482882

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7821528/

Abstract

BACKGROUND

Culicoides (Diptera: Ceratopogonidae) are vectors for many arboviruses. At least 20 species are considered as vectors or potential vectors of bluetongue virus (BTV) which cause bluetongue disease in ruminants. A BTV prevalence of 30-50% among cattle and goats in tropical southern Yunnan Province, China, prompted an investigation of the potential BTV vectors in this area.

METHODS

Culicoides were collected by light trapping at three sites in the tropical region of Yunnan Province. Species were identified based on morphology and DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in these specimens. Phylogenetic analysis was used to analyze the cox1 sequences of Culicoides specimens infected with BTV.

RESULTS

Approximately 67,000 specimens of Culicoides were collected, of which 748 were tested for the presence of BTV. Five specimens, including two of Culicoides jacobsoni, one of C. tainanus and two of C. imicola, were identified as infected with BTV. No specimens of C. (subgenus Trithecoides) or C. oxystoma tested were positive for BTV infection.

CONCLUSIONS

To our knowledge this is the first report of C. jacobsoni as a potential BTV vector and the fourth report of an association between C. tainanus and BTV, as well as the first direct evidence of an association between BTV and C. imicola in Asia. A fourth potential cryptic species within C. tainanus was identified in this study. Further analysis is required to confirm the importance of C. jacobsoni and C. tainanus in BTV epidemiology in Asia.

摘要

背景

蠓科（双翅目：蠓科）是许多虫媒病毒的载体。在中国云南省热带南部，至少有 20 种被认为是蓝舌病病毒（BTV）的载体或潜在载体，该病毒会导致反刍动物患蓝舌病。云南省热带地区牛和山羊中 BTV 的流行率为 30-50%，促使对该地区潜在 BTV 载体进行调查。

方法

在云南省热带地区的三个地点使用灯光诱捕法收集蠓。根据形态学和细胞色素 c 氧化酶亚基 1（cox1）的 DNA 序列鉴定物种。对这些标本进行 BTV RNA 存在的 PCR 和逆转录后的定量 PCR 检测。对感染 BTV 的蠓 cox1 序列进行系统发育分析。

结果

共采集了约 67000 只蠓标本，其中 748 只检测了 BTV 的存在。鉴定出 5 只标本感染了 BTV，其中包括 2 只蠓属 jacobsoni、1 只蠓属 tainanus 和 2 只蠓属 imicola。未检测到感染 BTV 的 C.（亚属 Trithecoides）或 C. oxystoma 标本。

结论

据我们所知，这是首次报道蠓属 jacobsoni 是潜在的 BTV 载体，也是第四次报道蠓属 tainanus 与 BTV 之间的关联，以及首次在亚洲直接证明 BTV 与蠓属 imicola 之间的关联。本研究还鉴定了 C. tainanus 中的第四个潜在隐种。需要进一步分析以确认 C. jacobsoni 和 C. tainanus 在亚洲 BTV 流行病学中的重要性。