Laboratory for Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.
Wageningen Food Safety Research, Wageningen University and Research, Postbus 230, 6700 AE, Wageningen, The Netherlands.
Sci Rep. 2021 Jan 22;11(1):2136. doi: 10.1038/s41598-021-81683-w.
Resistance plasmids play a crucial role in the transfer of antimicrobial resistance from the veterinary sector to human healthcare. In this study plasmids from foodborne Escherichia coli isolates with a known (ES)BL or tetracycline resistance were sequenced entirely with short- and long-read technologies to obtain insight into their composition and to identify driving factors for spreading. Resistant foodborne E. coli isolates often contained several plasmids coding for resistance to various antimicrobials. Most plasmids were large and contained multiple resistance genes in addition to the selected resistance gene. The majority of plasmids belonged to the IncI, IncF and IncX incompatibility groups. Conserved and variable regions could be distinguished in each of the plasmid groups. Clusters containing resistance genes were located in the variable regions. Tetracycline and (extended spectrum) beta-lactamase resistance genes were each situated in separate clusters, but sulphonamide, macrolide and aminoglycoside formed one cluster and lincosamide and aminoglycoside another. In most plasmids, addiction systems were found to maintain presence in the cell.
抗性质粒在将抗微生物药物耐药性从兽医部门转移到人类医疗保健中起着至关重要的作用。在这项研究中，对具有已知（ES）BL 或四环素耐药性的食源性病原体大肠杆菌分离株的质粒进行了全长测序，使用短读长和长读长技术，以深入了解其组成，并确定传播的驱动因素。耐药性食源性病原体大肠杆菌分离株通常含有多个编码对各种抗生素的耐药性的质粒。大多数质粒较大，除了选择的耐药基因外，还含有多个耐药基因。大多数质粒属于 IncI、IncF 和 IncX 不相容群。在每个质粒群中都可以区分保守区和可变区。含有耐药基因的簇位于可变区。四环素和（扩展谱）β-内酰胺酶耐药基因分别位于单独的簇中，但磺胺类、大环内酯类和氨基糖苷类形成一个簇，林可酰胺类和氨基糖苷类形成另一个簇。在大多数质粒中，发现成瘾系统有助于维持细胞内的存在。
