Área Académica de Medicina del Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca, Mexico.
Área Académica de Gerontología del Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca, Mexico.
Birth Defects Res. 2021 May;113(8):613-622. doi: 10.1002/bdr2.1873. Epub 2021 Jan 23.
Premature infants contribute to infant morbidity and mortality especially in low resource settings. Information on tocolytic and/or anti-inflammatory effects of several plant extracts, such as citral, could help prevent preterm birth cases and reduce the number of preterm infants. The aim of this study was to evaluate the in vitro tocolytic and anti-inflammatory effect of citral on myometrial tissues of the human uterus.
Myometrial samples from uteri obtained after hysterectomy were used in functional tests to evaluate the inhibitory effect of citral on PGF-2α induced contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to citral in human myometrial homogenates were measured by ELISAs. Forskolin was used as a positive control. The anti-inflammatory effect of citral was determined through the measurement of two pro-inflammatory cytokines, tumor necrosis factor-α (TNFα) and interleukin (IL)-1β, and the anti-inflammatory cytokine IL-10, in human myometrial explants stimulated with lipopolysaccharide (LPS).
Citral was able to induce a significant inhibition of PGF-2α induced contractions at the highest concentration level (p < .05). Citral caused a concentration-dependent increase in myometrial cAMP levels (p < .05) and a concentration-dependent decrease in LPS-induced TNFα and IL-1β production, while IL-10 production increased significantly (p < .05). The anti-inflammatory and tocolytic effects induced by citral could be associated with an increase in cAMP levels in human myometrial samples.
These properties place citral as a potentially safe and effective adjuvant agent in preterm birth cases, an obstetric and gynecological problem that requires urgent attention.
早产儿会导致婴儿发病率和死亡率增加，尤其是在资源匮乏的环境中。几种植物提取物，如柠檬醛，具有保胎和抗炎作用的信息，可能有助于预防早产病例并减少早产儿数量。本研究旨在评估柠檬醛对人子宫平滑肌组织的体外保胎和抗炎作用。
使用子宫切除术获得的子宫组织样本进行功能测试，以评估柠檬醛对 PGF-2α 诱导收缩的抑制作用。通过 ELISA 测量柠檬醛在人子宫匀浆中产生的细胞内环腺苷酸 (cAMP) 水平。福司可林用作阳性对照。通过测量脂多糖 (LPS) 刺激的人子宫外植体中的两种促炎细胞因子肿瘤坏死因子-α (TNFα) 和白细胞介素 (IL)-1β 以及抗炎细胞因子 IL-10，来确定柠檬醛的抗炎作用。
柠檬醛在最高浓度水平下能够显著抑制 PGF-2α 诱导的收缩（p<0.05）。柠檬醛引起浓度依赖性增加的子宫 cAMP 水平（p<0.05）和 LPS 诱导的 TNFα 和 IL-1β 产生的浓度依赖性降低，而 IL-10 产生显著增加（p<0.05）。柠檬醛诱导的抗炎和保胎作用可能与人类子宫组织样本中 cAMP 水平的增加有关。
这些特性使柠檬醛成为治疗早产病例的潜在安全有效的辅助药物，早产是一种需要紧急关注的妇产科问题。
