Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People's Republic of China.
Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China.
Biotechnol J. 2021 Aug;16(8):e2000575. doi: 10.1002/biot.202000575. Epub 2021 Jun 19.
Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01-1 is a broad-spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products is an effective method for bioprospecting, for which, genome manipulation tools are essential. We previously developed Pseudomonas-specific phage-derived homologous recombination systems for genetic engineering in four Pseudomonas species. Herein, we report the application of these recombineering systems in Pseudomonas parafulva CRS01-1, along with structural elucidation and bioactivity evaluation of natural products. The Pseudomonas recombineering toolbox established before in different four species is efficient for genome mining and bioactive metabolite discovery from more distant species.
微生物天然产物除了发挥其他功能外，在人类、动物和植物的疾病预防中也起着至关重要的作用。恶臭假单胞菌 CRS01-1 是一种存在于植物中的广谱拮抗细菌。然而，迄今为止尚未从该菌株中分离出天然产物。天然产物的相应生物合成基因簇是生物勘探的有效方法，为此，基因组操作工具是必不可少的。我们之前开发了针对四种假单胞菌的假单胞菌特异性噬菌体衍生同源重组系统，用于遗传工程。在此，我们报告了这些重组系统在恶臭假单胞菌 CRS01-1 中的应用，以及天然产物的结构阐明和生物活性评价。之前在四种不同物种中建立的假单胞菌重组工具盒对于从更远缘的物种进行基因组挖掘和生物活性代谢产物发现是有效的。
