788 Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, 161 Jesse Hill Jr. Drive, Atlanta, GA, 30302, USA.
Anal Bioanal Chem. 2021 Mar;413(7):1999-2006. doi: 10.1007/s00216-021-03168-z. Epub 2021 Jan 23.
Strict adherence to highly active antiretroviral therapy (HAART) is very important to improve the quality of life for HIV-positive patients to reduce new infections and determine treatment success. Azidothymidine (AZT) is an antiretroviral drug commonly used in HAART treatment. In this research, an "add, mix, and measure" assay was developed to detect AZT within minutes. Three different probes designed to release fluorophores when samples containing AZT are added were synthesized and characterized. The limit of detection to AZT in simulated urine samples was determined to be 4 μM in 5 min for one of the probes. This simple and rapid point-of-care test could potentially be used by clinicians and health care workers to monitor the presence of AZT in low resource settings.
严格遵循高效抗逆转录病毒疗法（HAART）对于提高 HIV 阳性患者的生活质量、减少新的感染和确定治疗成功非常重要。叠氮胸苷（AZT）是 HAART 治疗中常用的一种抗逆转录病毒药物。在这项研究中，开发了一种“添加、混合和测量”的测定方法，可在数分钟内检测 AZT。合成并表征了三种不同的探针，当添加含有 AZT 的样品时，这些探针会释放荧光团。其中一种探针在模拟尿液样本中的检测限在 5 分钟内达到 4 μM。这种简单快速的即时检测方法可能被临床医生和医疗保健工作者用于在资源匮乏的环境中监测 AZT 的存在。
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