SARS-CoV-2 5'UTR 茎环 5a 的 H、C 和 N 化学位移赋值。
H, C and N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2.
机构信息
Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
出版信息
Biomol NMR Assign. 2021 Apr;15(1):203-211. doi: 10.1007/s12104-021-10007-w. Epub 2021 Jan 23.
The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5'-untranslated region (5'-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5'-UUUCGU-3' hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive H, C and N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）是引发当前 COVID-19 大流行的病原体。它含有一条正链单链 RNA 基因组，属于贝塔冠状病毒属。SARS-CoV-2 基因组长 30kb，其 5'和 3'基因组末端是有潜力的抗病毒药物靶点。这些序列的大部分在贝塔冠状病毒属中高度保守，包含影响 RNA 翻译和复制的顺式作用 RNA 元件。31 个核苷酸（nt）长的高度保守的茎环 5a（SL5a）位于 5'非翻译区（5'-UTR）内，对病毒复制很重要。SL5a 具有富含 U 的非对称凸起，并被 5'-UUUCGU-3'六聚体环帽封闭，该六聚体环在茎环 5b（SL5b）中也有发现。在此，我们报告了 SL5a 的广泛的 H、C 和 N 共振归属，作为通过溶液 NMR 光谱进行深入结构研究的基础。
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