Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt, Germany.
Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt, Germany.
Biomol NMR Assign. 2022 Apr;16(1):17-25. doi: 10.1007/s12104-021-10053-4. Epub 2022 Feb 18.
The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5'- and 3'-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5'-untranslated region (5'-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5'-UUUCGU-3' hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive H, C and N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.
持续的呼吸道疾病 COVID-19 大流行是由 SARS-CoV-2（SCoV2）病毒引起的。SCoV2 是β冠状病毒属的一员。SCoV2 的 30kb 正链、单链 RNA 基因组具有高度保守的 5'-和 3'-基因组末端，这些末端包含参与病毒复制和翻译调节的结构顺式作用 RNA 元件。这些潜在抗病毒药物靶点的结构信息支持针对 COVID-19 的新型治疗药物的开发。在 5'-非翻译区（5'-UTR）内发现的高度保守的分支茎环 5（SL5）由一个基础茎和三个茎环组成，即 SL5a、SL5b 和 SL5c。SL5a 和 SL5b 都具有 5'-UUUCGU-3'六聚环，也存在于甲型冠状病毒中。在这里，我们报告了跨越 SL5b 和 SL5c（SL5b+c）的 37 个核苷酸（nt）长序列的 H、C 和 N 共振分配，作为通过溶液 NMR 光谱进行进一步深入结构研究的基础。
