Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA.
The Center for Biotechnology & Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Appl Biochem Biotechnol. 2021 May;193(5):1496-1512. doi: 10.1007/s12010-021-03492-1. Epub 2021 Jan 23.
In this study, Box-Behnken design was applied to optimize the initial concentrations of 4 cations for L-lactic acid production from fructose by homologous batch fermentation of Lactobacillus pentosus cells. The optimum initial cation concentrations were obtained as 6.542 mM Mg, 3.765 mM Mn, 2.397 mM Cu, and 3.912 mM Fe, respectively. The highest L-lactic acid yield and productivity were obtained as 0.935 ± 0.005 g/g fructose and 1.363 ± 0.021 g/(L × h), respectively, with a maximum biomass concentration of 7.97 ± 0.17 g/L. The effectiveness of the optimization by Box-Behnken design was confirmed based on the small errors between predicted results and experimental results shown as 0.3%, - 0.2%, and - 1.2%, respectively. The quadratic models with high accuracy and reliability can be applied to mathematically forecasted the fermentation performance. After the optimization, the lactic acid yield and productivity were significantly improved by 3.7% and 21.0%, respectively.
在这项研究中，应用 Box-Behnken 设计对戊糖乳杆菌同源分批发酵生产 L-乳酸的初始 4 种阳离子浓度进行优化。得到的最佳初始阳离子浓度分别为 6.542 mM Mg、3.765 mM Mn、2.397 mM Cu 和 3.912 mM Fe。获得的最高 L-乳酸产率和生产效率分别为 0.935±0.005 g/g 果糖和 1.363±0.021 g/（L·h），最大生物量浓度为 7.97±0.17 g/L。Box-Behnken 设计优化的有效性通过预测结果与实验结果之间的小误差（分别为 0.3%、-0.2%和-1.2%）得到了证实。这些高度准确和可靠的二次模型可用于对发酵性能进行数学预测。优化后，乳酸产率和生产效率分别显著提高了 3.7%和 21.0%。
