Department of Paper and Bioprocess Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA.
The Center for Biotechnology and Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Bioprocess Biosyst Eng. 2020 Jun;43(6):1071-1079. doi: 10.1007/s00449-020-02305-9. Epub 2020 Feb 8.
In this study, the immobilization technology was used to improve the LA yield and shorten the fermentation time. The optimum conditions to immobilize Lactobacillus pentosus ATCC 8041 cell were determined by Taguchi design L16 (4). The immobilized L. pentosus ATCC 8041 cells prepared by 2% sodium alginate (SA) and 6% polyvinyl alcohol (PVA) with the immobilization process by 0.10 M calcium chloride (CaCl) and 2.5% boric acid (HBO) had the best performance of LA yield at the temperature of 35 °C, which is significantly higher than that of L. pentosus ATCC 8041 free cells. These cells maintained the stable and efficient performance in 15 repeated batch fermentation, and they also have excellent mechanical strength to keep from breakage caused by cell growth and agitation.
在这项研究中，使用固定化技术来提高 LA 的产量并缩短发酵时间。通过 Taguchi 设计 L16（4）确定了固定化戊糖乳杆菌 ATCC 8041 细胞的最佳条件。用 2%海藻酸钠（SA）和 6%聚乙烯醇（PVA），通过 0.10 M 氯化钙（CaCl）和 2.5%硼酸（HBO）的固定化过程制备的固定化戊糖乳杆菌 ATCC 8041 细胞在 35°C 的温度下具有最佳的 LA 产量性能，明显高于戊糖乳杆菌 ATCC 8041 游离细胞。这些细胞在 15 次重复分批发酵中保持稳定且高效的性能，并且它们还具有优异的机械强度，以防止因细胞生长和搅拌而导致的破裂。
