Wang Jianfei, Huang Jiaqi, Jiang Shaoming, Zhang Jing, Zhang Quanquan, Ning Yuchen, Fang Mudannan, Liu Shijie
Department of Paper and Bioprocess Engineering, SUNY College of Environmental Science and Forestry, Syracuse, USA.
The Center for Biotechnology & Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, USA.
Biotechnol Appl Biochem. 2021 Aug;68(4):809-822. doi: 10.1002/bab.1994. Epub 2020 Aug 13.
Parametric optimization always plays important roles in bioengineering systems to obtain a high product yield under the proper conditions. The parametric conditions of lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells was optimized by the Box-Behnken design. The highest l-lactic acid yield was obtained as 0.836 ± 0.003 g/g glucose with the productivity of 0.906 ± 0.003 g/(L × H) under the optimum conditions of 34.7 °C, pH 6.2, 148 rpm agitation speed, and 9.3 g/L nitrogen source concentration determined by quadratic response surface with high accuracy. The adequate kinetic models of cell growth rate, lactic production rate, and glucose consumption rate were also established to describe the fermentation behavior of L. pentosus cells with the correlation coefficients of 09985, 0.9990, and 0.9989, respectively.
参数优化在生物工程系统中始终发挥着重要作用，以便在适当条件下获得高产品产量。采用Box-Behnken设计对戊糖乳杆菌细胞同源分批发酵生产乳酸的参数条件进行了优化。在34.7℃、pH 6.2、148 rpm搅拌速度和9.3 g/L氮源浓度的最佳条件下，通过高精度二次响应面确定，获得了最高的L-乳酸产量，为0.836±0.003 g/g葡萄糖，生产率为0.906±0.003 g/(L×h)。还建立了细胞生长速率、乳酸生成速率和葡萄糖消耗速率的适当动力学模型，以描述戊糖乳杆菌细胞的发酵行为，相关系数分别为0.9985、0.9990和0.9989。
