Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.
Agricultural Chemistry Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.
J Appl Microbiol. 2021 Sep;131(3):1378-1390. doi: 10.1111/jam.15016. Epub 2021 Feb 9.
The present study evaluated, for the first time, the inhibitory effects of the filtrate of Purpureocillium lilacinum against Penicillium digitatum.
No direct contact between P. lilacinum and P. digitatum was observed during the dual culture test and the inhibition zone was 6·1 mm. The filtrate of P. lilacinum completely inhibited P. digitatum growth and spore germination at the concentration of 64%. The filtrate increased the permeability of the cell membrane and the content of MDA in P. digitatum. The ergosterol content in P. digitatum was strongly inhibited at 32% by 81·1%. The green mould incidence and severity in filtrate-treated fruit at 64% were 71·7 and 80·7% lower than in the control, respectively. The filtrate enhanced the activity of PAL, PPO and POD enzymes in orange fruit. The POD and PAL gene expression levels were significantly upregulated in the fruit treated with the filtrate.
This study indicated that the antifungal mechanism of P. lilacinum filtrate against P. digitatum is mainly by the damage of the fungal cell membrane and its components.
This work provides the pioneer evidence on the application of P. lilacinum filtrate as a novel biocontrol agent for orange green mould.
本研究首次评价了紫色毛壳菌滤液对柑橘绿霉的抑制作用。
在双层培养试验中未观察到紫色毛壳菌与柑橘绿霉的直接接触，抑菌带为 6.1mm。紫色毛壳菌滤液在 64%的浓度下完全抑制了柑橘绿霉的生长和孢子萌发。滤液增加了细胞膜的通透性和 MDA 的含量。在 32%的浓度下，紫色毛壳菌中的麦角固醇含量强烈抑制了 81.1%。在 64%的滤液处理过的果实中，青霉病的发病率和严重度分别比对照低 71.7%和 80.7%。滤液增强了柑橘果实中 PAL、PPO 和 POD 酶的活性。经滤液处理的果实中 POD 和 PAL 基因表达水平显著上调。
本研究表明，紫色毛壳菌滤液对柑橘绿霉的抑菌机制主要是通过破坏真菌细胞膜及其成分。
这项研究为紫色毛壳菌滤液作为一种新型的防治柑橘绿霉的生物防治剂提供了开创性的证据。
