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鉴定丁香属植物研究中合适的内参基因

Identification of suitable reference genes for studies of Syringa pinnatifolia Hemsl.

机构信息

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

School of Pharmacy, Jiangsu University, Zhenjiang, China.

出版信息

FEBS Open Bio. 2021 Apr;11(4):1041-1053. doi: 10.1002/2211-5463.13097. Epub 2021 Feb 26.

DOI:10.1002/2211-5463.13097
PMID:33484622
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8016119/
Abstract

Syringa pinnatifolia Hemsl. (Oleaceae) is a species of shrub with a limited distribution in China. Several chemical compounds with pharmacological effects have been isolated from S. pinnatifolia, including new lignans and sesquiterpenes. Studies of gene expression in this species require the identification of suitable reference genes that are stably expressed under different conditions and in different tissues. To identify candidate reference genes, here we used the geNorm, NormFinder, and BestKeeper algorithms to analyze the stability of 12 candidate genes. The geometric mean of the rankings generated with these algorithms was used to obtain a comprehensive ranking. TBP and PP2A were found to be appropriate reference genes for calli treated with different external stimuli, and TIP41 and TBP were found to be appropriate reference genes in differentiated tissues. When calli and differentiated tissues were considered together, TBP and TIP41 were found to be the most reliable reference genes. The selected genes were validated by analysis of HMGR expression in calli and differentiated tissues. This study is the first to screen candidate reference genes in the genus Syringa and could help guide future molecular studies in this genus.

摘要

丁香属（Syringa）植物是木樨科的一个灌木种，在中国的分布范围有限。已从丁香属植物中分离出多种具有药理作用的化合物，包括新的木脂素和倍半萜。该物种的基因表达研究需要鉴定在不同条件和不同组织中稳定表达的合适参考基因。为了鉴定候选参考基因，我们使用 geNorm、NormFinder 和 BestKeeper 算法分析了 12 个候选基因的稳定性。使用这些算法生成的排名的几何平均值用于获得综合排名。结果发现 TBP 和 PP2A 是对外界刺激处理的愈伤组织的合适参考基因，TIP41 和 TBP 是分化组织的合适参考基因。当同时考虑愈伤组织和分化组织时，TBP 和 TIP41 是最可靠的参考基因。通过对愈伤组织和分化组织中 HMGR 表达的分析验证了所选基因。这项研究首次筛选了丁香属中的候选参考基因，可为该属的未来分子研究提供指导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/7caeb9ac4f3e/FEB4-11-1041-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/8370ff6aeef3/FEB4-11-1041-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/f033ac12948c/FEB4-11-1041-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/3102d3964c42/FEB4-11-1041-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/3eab41b12772/FEB4-11-1041-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/010ddcba27d8/FEB4-11-1041-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/863caa134520/FEB4-11-1041-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/7caeb9ac4f3e/FEB4-11-1041-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/8370ff6aeef3/FEB4-11-1041-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/f033ac12948c/FEB4-11-1041-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/3102d3964c42/FEB4-11-1041-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/3eab41b12772/FEB4-11-1041-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/010ddcba27d8/FEB4-11-1041-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/863caa134520/FEB4-11-1041-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1915/8016119/7caeb9ac4f3e/FEB4-11-1041-g002.jpg

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