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琼脂品牌和微量元素对 Granulicella sp.(酸杆菌门)生长优化的影响。

The influence of agar brands and micronutrients in the growth optimization of Granulicella sp. (Acidobacteriota).

机构信息

Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Droevendaalsesteeg 10, 6708, PB, Wageningen, the Netherlands.

Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Droevendaalsesteeg 10, 6708, PB, Wageningen, the Netherlands; Institute of Environmental Biology, Ecology and Biodiversity, Utrecht University, 3584, CH, Utrecht, the Netherlands.

出版信息

J Microbiol Methods. 2021 Feb;181:106148. doi: 10.1016/j.mimet.2021.106148. Epub 2021 Jan 20.

Abstract

Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should be carefully considered for Acidobacteriota strains growth and microbial isolation.

摘要

酸杆菌门在土壤中丰度很高,但可培养的代表物种较少。试剂的纯度会影响实验室条件下微生物的生长和成功分离。在这里,我们研究了不同琼脂品牌对培养基中微生物生长的影响,并主张在培养酸杆菌门菌株和微生物分离时,应仔细考虑琼脂的来源。

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