用于金黄色葡萄球菌生物膜培养的琼脂和琼脂糖会影响氟喹诺酮类药物的耐受性。
Agar and agarose used for Staphylococcus aureus biofilm cultivation impact fluoroquinolone tolerance.
机构信息
Department of Molecular Biology & Biophysics, UConn Health, Farmington, CT 06032, United States.
出版信息
J Appl Microbiol. 2024 Aug 5;135(8). doi: 10.1093/jambio/lxae191.
AIMS
Staphylococcus aureus is an opportunistic pathogen whose treatment is further complicated by its ability to form biofilms. In this study, we examine the impact of growing S. aureus biofilms on different polymerizing surfaces, specifically agar and agarose, on the pathogen's tolerance to fluoroquinolones.
METHODS AND RESULTS
Biofilms of two methicillin-resistant strains of S. aureus were grown on agar or agarose in the presence of the same added nutrients, and their antibiotic susceptibility to two fluoroquinolones, moxifloxacin (MXF) and delafloxacin (DLX), were measured. We also compared the metabolism and extracellular polymeric substances (EPS) production of biofilms that were grown on agar and agarose.
CONCLUSIONS
Biofilms that were grown on agarose were consistently more susceptible to antibiotics than those grown on agar. We found that in biofilms that were grown on agar, extracellular protein composition was higher, and adding EPS to agarose-grown biofilms increased their tolerance to DLX to levels that were comparable to agar-grown biofilms.
目的
金黄色葡萄球菌是一种机会致病菌，其形成生物膜的能力使其治疗更加复杂。在这项研究中，我们研究了在琼脂和琼脂糖等不同聚合表面上生长的金黄色葡萄球菌生物膜对氟喹诺酮类药物的耐受性的影响。
方法和结果
在添加相同营养物质的情况下，在琼脂或琼脂糖上生长了两种耐甲氧西林金黄色葡萄球菌的生物膜，并测量了它们对两种氟喹诺酮类药物，莫西沙星（MXF）和德拉沙星（DLX）的抗生素敏感性。我们还比较了在琼脂和琼脂糖上生长的生物膜的代谢和细胞外聚合物（EPS）的产生。
结论
在琼脂糖上生长的生物膜比在琼脂上生长的生物膜对抗生素更敏感。我们发现，在琼脂上生长的生物膜中，细胞外蛋白质组成更高，并且向琼脂糖上生长的生物膜中添加 EPS 可将其对 DLX 的耐受性提高到与琼脂上生长的生物膜相当的水平。
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