S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2951-2959. doi: 10.1099/ijs.0.021824-0. Epub 2010 Jan 29.
Five strains of strictly aerobic, heterotrophic bacteria that form pink-red colonies and are capable of hydrolysing pectin, xylan, laminarin, lichenan and starch were isolated from acidic Sphagnum peat bogs and were designated OB1010(T), LCBR1, TPB6011(T), TPB6028(T) and TPO1014(T). Cells of these isolates were Gram-negative, non-motile rods that produced an amorphous extracellular polysaccharide-like substance. Old cultures contained spherical bodies of varying sizes, which represent starvation forms. Cells of all five strains were acidophilic and psychrotolerant, capable of growth at pH 3.0-7.5 (optimum pH 3.8-4.5) and at 2-33°C (optimum 15-22°C). The major fatty acids were iso-C(15 : 0), C(16 : 0) and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH). The major menaquinone detected was MK-8. The pigments were carotenoids. The genomic DNA G+C contents were 57.3-59.3 mol%. The five isolates were found to be members of subdivision 1 of the phylum Acidobacteria and displayed 95.3-98.9 % 16S rRNA gene sequence similarity to each other. The closest described relatives to strains OB1010(T), LCBR1, TPB6011(T), TPB6028(T), and TPO1014(T) were members of the genera Terriglobus (94.6-95.8 % 16S rRNA gene sequence similarity) and Edaphobacter (94.2-95.4 %). Based on differences in cell morphology, phenotypic characteristics and hydrolytic capabilities, we propose a novel genus, Granulicella gen. nov., containing four novel species, Granulicella paludicola sp. nov. with type strain OB1010(T) (=DSM 22464(T) =LMG 25275(T)) and strain LCBR1, Granulicella pectinivorans sp. nov. with type strain TPB6011(T) (=VKM B-2509(T) =DSM 21001(T)), Granulicella rosea sp. nov. with type strain TPO1014(T) (=DSM 18704(T) =ATCC BAA-1396(T)) and Granulicella aggregans sp. nov. with type strain TPB6028(T) (=LMG 25274(T) =VKM B-2571(T)).
从酸性泥炭沼泽中分离到 5 株严格需氧异养细菌，它们形成粉红色到红色的菌落，能够水解果胶、木聚糖、昆布多糖、石莼聚糖和淀粉，将它们分别命名为 OB1010(T)、LCBR1、TPB6011(T)、TPB6028(T)和 TPO1014(T)。这些分离株的细胞为革兰氏阴性、非运动棒状，产生无定形细胞外多糖样物质。旧培养物中含有不同大小的球形体，代表饥饿形式。所有 5 株菌均为嗜酸、耐冷，能够在 pH 3.0-7.5（最佳 pH 3.8-4.5）和 2-33°C（最佳 15-22°C）下生长。主要脂肪酸为 iso-C(15：0)、C(16：0)和 sum feature 3（C(16：1)ω7c 和/或 iso-C(15：0) 2-OH）。检测到的主要类异戊二烯醌为 MK-8。色素为类胡萝卜素。基因组 DNA G+C 含量为 57.3-59.3 mol%。这 5 株菌被发现属于酸杆菌门的 1 亚门，彼此之间的 16S rRNA 基因序列相似性为 95.3-98.9%。与菌株 OB1010(T)、LCBR1、TPB6011(T)、TPB6028(T)和 TPO1014(T)最接近的描述性亲缘关系的属成员为 Terriglobus（94.6-95.8% 16S rRNA 基因序列相似性）和 Edaphobacter（94.2-95.4% 16S rRNA 基因序列相似性）。基于细胞形态、表型特征和水解能力的差异，我们提出了一个新属，Granulicella gen. nov.，包含四个新种，Granulicella paludicola sp. nov.，其模式菌株为 OB1010(T)=DSM 22464(T)=LMG 25275(T)和 LCBR1，Granulicella pectinivorans sp. nov.，其模式菌株为 TPB6011(T)=VKM B-2509(T)=DSM 21001(T)，Granulicella rosea sp. nov.，其模式菌株为 TPO1014(T)=DSM 18704(T)=ATCC BAA-1396(T)和 Granulicella aggregans sp. nov.，其模式菌株为 TPB6028(T)=LMG 25274(T)=VKM B-2571(T)。
