School of Basic Medical Science, Cheeloo College of Medicine, Shandong University, Jinan & Shenzhen Hospital, Cancer Hospital of the Chinese Academy of Medical Sciences, Shenzhen, China.
Cancer Therapy and Research Center, Shandong Provincial Hospital, Jinan, Shandong, 250021, China.
Microb Pathog. 2021 Feb;151:104747. doi: 10.1016/j.micpath.2021.104747. Epub 2021 Jan 21.
The diagnostic and therapeutic role of intestinal microbiota in gastric carcinogenesis remains unclear. In this study, feces from gastric cancer patients and healthy people were sequenced for microbiota analysis, and the correlation between fecal bacteria and the occurrence of gastric cancer was explored. The β-diversity results showed that microbial compositions varied between gastric cancer patients and healthy people. Interestingly, the dissection of microbial structure revealed that all facultative anaerobic genera with relatively high abundances expanded significantly in gastric cancer patients. The succeeding correlation analysis demonstrated a distorted interaction of intestinal bacteria in gastric cancer. The application of some differential bacteria, Desulfovibrio, Escherichia, Faecalibacterium or Oscillospira, as biomarkers to predict gastric cancer could all reach an accuracy of 0.900 or above. The shift in Desulfovibrio was specifically verified by qPCR in newly collected fecal samples, and the patients with stage IV gastric cancer were identified to have significantly more Desulfovibrio than those with stage I, II and III gastric cancer. The possible role of Desulfovibrio in gastric cancer was assessed with HS-treated HT-29 cells, and the results showed that HS induced NO, IL-1β and IL-18 production, which is important for inflammation promotion and can be delivered through the bloodstream. This study suggests a correlation of intestinal microbiota and the development of gastric cancer.
肠道微生物群在胃癌发生中的诊断和治疗作用尚不清楚。在这项研究中，对胃癌患者和健康人的粪便进行了微生物组分析，探讨了粪便细菌与胃癌发生的相关性。β多样性结果表明，胃癌患者和健康人群的微生物组成存在差异。有趣的是，微生物结构的剖析表明，所有丰度较高的兼性厌氧属在胃癌患者中均显著扩张。随后的相关性分析表明，胃癌患者的肠道细菌相互作用发生了扭曲。一些差异细菌，如脱硫弧菌、大肠杆菌、粪杆菌或颤螺旋菌，作为预测胃癌的生物标志物，其准确性均达到 0.900 或以上。通过对新收集的粪便样本进行 qPCR 验证，发现Ⅳ期胃癌患者的脱硫弧菌数量明显多于Ⅰ、Ⅱ和Ⅲ期胃癌患者。通过 HS 处理的 HT-29 细胞评估脱硫弧菌在胃癌中的可能作用，结果表明 HS 诱导了 NO、IL-1β和 IL-18 的产生，这对炎症的促进很重要，并且可以通过血液传递。本研究提示肠道微生物群与胃癌的发展存在相关性。
