Department of Oral Disease Research, National Center for Geriatrics and Gerontology, 7-430 Morioka-cho, Obu City, Aichi, 474-8511, Japan.
Department of Laboratory of Experimental Animals, National Center for Geriatrics and Gerontology, 7-430 Morioka-cho, Obu City, Aichi, 474-8511, Japan.
J Oral Biosci. 2021 Mar;63(1):58-65. doi: 10.1016/j.job.2020.12.003. Epub 2021 Jan 21.
Tooth bleaching has become one of the most frequently requested esthetic procedures in dental practice. A side effect of bleaching is gingival irritation. This study examined the efficacy of propolis to treat gingival irritation caused by bleaching in vivo and in vitro.
Gingival irritation was mimicked by a mild burn injury to oral mucosa in young (10 week old) and aged (18 month old) mice. Propolis ointment was immediately applied to the burn area. After 24 h, gingiva was collected to determine the efficacy of propolis by hematoxylin and eosin staining and real-time polymerase chain reaction (PCR).
Topical application of propolis ointment reduced the infiltration of inflammatory cells at irritated sites and promoted the repair of the mucosal epithelium in young and aged mice. It also suppressed the expression of IL-1β and TNF-α and increased keratin 1 and 5 expression in the irritated gingiva. Propolis suppressed an increase in IL-1β and TNF-α upon stimulation with HO in young and SA-β-gal-expressing senescent human gingival fibroblasts (HGFs) cultures.
Propolis may be effective for mucosal repair in gingival irritation as it suppresses the expression of proinflammatory cytokines and promotes keratin expression.
牙齿漂白已成为口腔临床最常要求的美容程序之一。漂白的副作用之一是牙龈刺激。本研究探讨了蜂胶治疗漂白引起的牙龈刺激的疗效，分别在体内和体外进行了研究。
通过对年轻（10 周龄）和老年（18 月龄）小鼠的口腔黏膜进行轻度烧伤来模拟牙龈刺激。立即将蜂胶软膏涂抹在烧伤区域。24 小时后，收集牙龈，通过苏木精和伊红染色和实时聚合酶链反应（PCR）来确定蜂胶的疗效。
蜂胶软膏的局部应用减少了炎症细胞在受刺激部位的浸润，并促进了年轻和老年小鼠黏膜上皮的修复。它还抑制了炎性细胞因子 IL-1β 和 TNF-α 的表达，并增加了受刺激牙龈中角蛋白 1 和 5 的表达。蜂胶抑制了 HO 刺激下年轻和 SA-β-gal 表达的衰老人牙龈成纤维细胞（HGFs）培养物中 IL-1β 和 TNF-α 的增加。
蜂胶可能对牙龈刺激的黏膜修复有效，因为它抑制了促炎细胞因子的表达并促进了角蛋白的表达。
