Hacettepe University, Department of Restorative Dentistry, Ankara, Turkey.
Oper Dent. 2011 Nov-Dec;36(6):572-80. doi: 10.2341/10-058-C. Epub 2011 Sep 13.
This study aimed to evaluate the color change and clinical periodontal parameters and to analyze the interleukin–1 beta (IL-1β) and interleukin-10 (IL-10) levels in gingival crevicular fluid (GCF) of patients treated with different bleaching systems.
According to pre-established criteria, 30 healthy volunteers were selected and randomly divided into three groups (n=10): G1, home bleaching (Opalescence 35% Carbamide Peroxide, CP); G2, chemically activated office bleaching (Opalescence Xtra Boost 38% Hydrogen Peroxide, HP); G3, light-activated office bleaching (Opalescence Xtra 35% HP). Treatments were applied according to the manufacturer's recommendations. After shade evaluation, clinical periodontal parameters were evaluated as follows: gingival index (GI), plaque index (PI), and bleeding on probing (BOP). GCF were collected from six maxillary sites per patient at baseline (T0), one day (T1) after bleaching treatments, and 15 days (T2) after bleaching treatments and analyzed for IL-1β and IL-10 by enzyme-linked immunosorbent assay. Data were subjected to statistical analysis (p<0.05).
Spectrophotometer readings exhibited significant differences among the groups (p<0.05). The ΔE values (color change) of G3 were statistically higher than the other groups (p<0.05). The PI of G3 after 15 days was significantly higher than the PI of G2 after 15 days (p<0.05). The GI of G2 was lower than that of G1 and G3 before bleaching (p<0.05). According to BOP, no significant differences were found among the groups at any time intervals (p>0.05). In G3, the total amount of IL-1β after 15 days was higher than the amount before bleaching (p<0.05). The IL-10 total amount and concentration levels did not exhibit any significant differences among the groups or by time (p>0.05).
Home and chemically activated bleaching systems could be considered as safer in tooth whitening and maintaining gingival health when compared with a light-activated bleaching system, which might lead to increased proinflammatory cytokine (IL-1β).
本研究旨在评估不同漂白系统治疗患者的颜色变化和临床牙周参数，并分析龈沟液（GCF）中的白细胞介素-1β（IL-1β）和白细胞介素-10（IL-10）水平。
根据预先确定的标准，选择 30 名健康志愿者，并将其随机分为三组（n=10）：G1，家庭漂白（Opalescence 35% Carbamide Peroxide，CP）；G2，化学激活诊室漂白（Opalescence Xtra Boost 38% Hydrogen Peroxide，HP）；G3，光激活诊室漂白（Opalescence Xtra 35% HP）。根据制造商的建议进行治疗。在进行颜色评估后，评估以下临床牙周参数：牙龈指数（GI）、菌斑指数（PI）和探诊出血（BOP）。从每位患者的六个上颌部位采集 GCF，在基线（T0）、漂白治疗后一天（T1）和漂白治疗后 15 天（T2）进行分析，并通过酶联免疫吸附试验分析 IL-1β 和 IL-10。数据进行统计学分析（p<0.05）。
分光光度计读数显示组间存在显著差异（p<0.05）。G3 的 ΔE 值（颜色变化）明显高于其他组（p<0.05）。G3 组在 15 天时的 PI 明显高于 G2 组在 15 天时的 PI（p<0.05）。G2 组的 GI 在漂白前低于 G1 组和 G3 组（p<0.05）。根据 BOP，在任何时间间隔均未发现组间存在显著差异（p>0.05）。在 G3 组中，15 天后的总 IL-1β 量高于漂白前的量（p<0.05）。各组和各时间点的 IL-10 总量和浓度均无显著差异（p>0.05）。
与光激活漂白系统相比，家庭和化学激活漂白系统在牙齿美白和维持牙龈健康方面可能更为安全，因为前者可能导致促炎细胞因子（IL-1β）增加。
