Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan.
Department of Chemistry, IPGGPG College of Commerce, Haldwani 263139, Uttarakhand, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15;251:119456. doi: 10.1016/j.saa.2021.119456. Epub 2021 Jan 9.
Hydrazine or hydrazine hydrate (NH·HO) is a potential neurotoxin and has several mutagenic effects in physiological systems. Therefore, the development of synthetic organic probes that are sensitive and selective to hydrazine is of tremendous importance. Unfortunately, however, the hydrazine-selective sensing probes that rely upon minimum usage of the organic solvents (≤5%, v/v) are still rarer. In this work, an ion-pair-like mono acetate derivative of bromophenol blue has been developed as a fairly selective ratiometric probe for the naked-eye recognition of hydrazine in a solution of tris buffer and EtOH (19:1, v/v) at physiological pH. The chromogenic signalling relies upon hydrazine-induced cleavage of an ester moiety of the probe to its resonance stabilized quinonoid form, resulting in momentous variations in its spectrophotometric profile. Meanwhile, the colour of the probe solution changed from mustard yellow to blue within few minutes. This sensing assay could be successfully applied in the recognition of hydrazine in real environmental and pharmaceutical samples with satisfactory recoveries. Given the cost-effectiveness, simplicity and versatility, for instance, direct analysis of colorimetric probes, it is reasonable to propose that the present method can serve as a complementary method for prompt inspection of hydrazine in boiler feed water.
水合肼（NH·HO）是一种潜在的神经毒素，在生理系统中有多种诱变作用。因此，开发对水合肼敏感和选择性的合成有机探针非常重要。然而，遗憾的是，仍然很少有依赖于有机溶剂（≤5%，v/v）最小用量的水合肼选择性传感探针。在这项工作中，溴酚蓝的单乙酸酯类似物离子对衍生物已被开发为一种相当选择性的比色探针，用于在生理 pH 值下 tris 缓冲液和 EtOH（19:1，v/v）溶液中对水合肼进行裸眼识别。显色信号依赖于水合肼诱导探针酯部分的裂解，形成其共振稳定的醌式形式，导致其分光光度特性发生重大变化。同时，探针溶液的颜色在几分钟内从芥末黄变为蓝色。该传感测定法可成功应用于实际环境和药物样品中对水合肼的识别，回收率令人满意。鉴于成本效益、简单性和多功能性，例如直接分析比色探针，因此可以合理地提出，本方法可以作为锅炉给水中水合肼快速检测的补充方法。
