Palanisamy Jayasudha, Rajagopal Rajakrishnan, Alfarhan Ahmed
Department of Chemistry, Subramanya College of Arts and Science, Tamilnadu, Palani, 624618, India.
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
J Fluoresc. 2024 Jun 27. doi: 10.1007/s10895-024-03796-5.
A newly synthesized naphthalimide-based fluorophore probe NIA was used to detect hydrazine. This probe, based on the Gabriel mechanism exhibited a highly sensitive revealing of hydrazine in naked eyes colorimetric as well as fluorescent recognition against other amines in an aqueous solution in DMSO - HEPES buffer. When hydrazine hydrate was added to the probe NIA, the absorption was red shifted from 403 nm to 520 nm. The titration studies by adding hydrazine to show two apparent isosbestic points found at 358 and 450 nm, respectively. Further, investigation of emission spectra upon addition of hydrazine hydride the emission peak at 493 nm gradually decreased up to 2.4 equiv. and when increasing the hydrazine hydride concentration from 2.4 equiv. to 4.4 equiv., the fluorescence intensity increased at 530 nm. which is exhibiting a raised ratiometric emission intensity at 530 nm. Further investigation of the selectivity of probe NIA revealed colorimetric and fluorimetric responses to interferences with other test amines. H NMR and HR-mass proved the Gabriel mechanism bath for detecting hazardous hydrazine by probe NIA. This probe NIA allowed the rapid and ultrasensitive detection of hydrazine hydride with a low detection limit of 0.26 nM. In view of the outstanding properties, probe NIA has been effectively performed to detect hydrazine using various techniques, including a test kit, silica support, and actual environmental water samples.
一种新合成的基于萘酰亚胺的荧光团探针NIA被用于检测肼。该探针基于盖布瑞尔机理，在DMSO - HEPES缓冲水溶液中，对肼表现出高灵敏度的肉眼比色以及荧光识别，可区分其他胺类。当水合肼添加到探针NIA中时，吸收峰从403 nm红移至520 nm。通过添加肼进行的滴定研究表明，分别在358和450 nm处发现了两个明显的等吸收点。此外，添加氢化肼后发射光谱的研究表明，493 nm处的发射峰在加入2.4当量氢化肼之前逐渐降低，当氢化肼浓度从2.4当量增加到4.4当量时，530 nm处的荧光强度增加，即在530 nm处呈现出升高的比率发射强度。对探针NIA选择性的进一步研究揭示了其对其他测试胺类干扰的比色和荧光响应。1H NMR和高分辨质谱证明了探针NIA检测有害肼的盖布瑞尔机理。该探针NIA能够快速、超灵敏地检测氢化肼，检测限低至0.26 nM。鉴于其优异的性能，探针NIA已通过多种技术有效地用于检测肼，包括测试试剂盒、硅胶载体和实际环境水样。
