用于检测外来反刍动物恶性卡他热的非洲马瘟病毒抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for the detection of antibodies to the alcelaphine herpesvirus of malignant catarrhal fever in exotic ruminants.
作者信息
Wan S K, Castro A E, Heuschele W P, Ramsay E C
机构信息
Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
出版信息
Am J Vet Res. 1988 Feb;49(2):164-8.
An ELISA for antibodies to the alcelaphine herpesvirus-1 of malignant catarrhal fever was developed. Of sera that represented 42 exotic ruminant species, 216 were evaluated by the ELISA and a virus-neutralization test. A significant correlation (r = 0.564, P less than 0.001, n = 216) between the ELISA and virus-neutralization test results was found. Of the sera having positive test results by virus neutralization, 86.1% also had positive results by the ELISA, and of the sera having negative test results by virus neutralization, 83.9% also had negative results by the ELISA. The presence of antibody, as measured by the ELISA, correlated with clinical signs of malignant catarrhal fever and the isolation of herpesvirus.
开发了一种用于检测恶性卡他热的非洲马瘟病毒1型抗体的酶联免疫吸附测定（ELISA）。对代表42种外来反刍动物物种的血清进行检测，采用ELISA和病毒中和试验对216份血清进行了评估。ELISA和病毒中和试验结果之间存在显著相关性（r = 0.564，P < 0.001，n = 216）。在病毒中和试验呈阳性的血清中，86.1%的血清ELISA检测也呈阳性；在病毒中和试验呈阴性的血清中，83.9%的血清ELISA检测也呈阴性。通过ELISA检测到的抗体的存在与恶性卡他热的临床症状和疱疹病毒的分离相关。
Zotero 插件