筛选过氧化物酶以优化葡萄糖氧化酶-过氧化物酶-原位化学氧化级联反应体系及其在黑色素脱色中的应用。
Screening of perhydrolases to optimize glucose oxidase-perhydrolase-in situ chemical oxidation cascade reaction system and its application in melanin decolorization.
机构信息
National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China; College of Life Sciences, Fujian Normal University (Qishan Campus), Fuzhou, 350117, China.
National & Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Ministry of Education, Fujian Normal University, Fuzhou, 350117, China; College of Life Sciences, Fujian Normal University (Qishan Campus), Fuzhou, 350117, China; Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Fujian Normal University, Fuzhou, 350117, China.
出版信息
J Biotechnol. 2021 Feb 20;328:106-114. doi: 10.1016/j.jbiotec.2021.01.013. Epub 2021 Jan 21.
A novel glucose oxidase (GOD)-perhydrolase-in situ chemical oxidation (ISCO) cascade reaction system was designed, optimized, and verified the operation feasibility in this research. Among the determined four perhydrolases, acyltransferase from Mycobacterium smegmatis (MsAcT) displayed the highest specific activity for perhydrolysis reaction (76.4 U/mg) and the lowest K value to hydrogen peroxide (13.9 mmol/L). GOD-MsAcT cascade reaction system also displayed high catalytic efficiency. Under the optimal parameters (50:1 activity unit ratio of GOD to MsAcT, pH 8.0, 50 mmol/L of β-d-glucose, and 15 mmol/L of glyceryl triacetate), the melanin decolorization rate using GOD-MsAcT-ISCO cascade reaction system reached 86.8 %. Kinetics of GOD-MsAcT-ISCO cascade reaction system for melanin decolorization fitted the kinetic model of Boltzmann sigmoid. As a substitutive skin whitening technology, GOD-MsAcT-ISCO cascade reaction system displayed an excellent application prospect.
本研究设计、优化并验证了一种新型葡萄糖氧化酶（GOD）-过氢化酶-原位化学氧化（ISCO）级联反应系统。在确定的四种过氢化酶中，来自耻垢分枝杆菌（Mycobacterium smegmatis）的酰基转移酶（MsAcT）对过水解反应表现出最高的比活性（76.4 U/mg）和最低的过氧化氢 K 值（13.9 mmol/L）。GOD-MsAcT 级联反应系统也表现出很高的催化效率。在最佳参数（GOD 与 MsAcT 的活性单位比为 50:1，pH8.0，β-d-葡萄糖浓度为 50mmol/L，甘油三乙酸酯浓度为 15mmol/L）下，GOD-MsAcT-ISCO 级联反应系统对黑色素的脱色率达到 86.8%。GOD-MsAcT-ISCO 级联反应系统对黑色素脱色的动力学符合 Boltzmann 型的动力学模型。作为一种替代皮肤美白技术，GOD-MsAcT-ISCO 级联反应系统显示出了极好的应用前景。
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