Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay.
Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay; Laboratorio Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo 11600, Uruguay.
Genomics. 2021 Mar;113(2):620-632. doi: 10.1016/j.ygeno.2021.01.005. Epub 2021 Jan 21.
Most parasitic flatworms go through different life stages with important physiological and morphological changes. In this work, we used a transcriptomic approach to analyze the main life-stages of the model tapeworm Hymenolepis microstoma (eggs, cysticercoids, and adults). Our results showed massive transcriptomic changes in this life cycle, including key gene families that contribute substantially to the expression load in each stage. In particular, different members of the cestode-specific hydrophobic ligand-binding protein (HLBP) family are among the most highly expressed genes in each life stage. We also found the transcriptomic signature of major metabolic changes during the transition from cysticercoids to adult worms. Thus, this work contributes to uncovering the gene expression changes that accompany the development of this important cestode model species, and to the best of our knowledge represents the first transcriptomic study with robust replicates spanning all of the main life stages of a tapeworm.
大多数寄生扁形动物经历不同的生命阶段，伴随着重要的生理和形态变化。在这项工作中，我们使用转录组学方法来分析模型绦虫细粒棘球绦虫（虫卵、囊尾蚴和成虫）的主要生命阶段。我们的结果表明，在这个生命周期中发生了大规模的转录组变化，包括对每个阶段的表达负荷有重要贡献的关键基因家族。特别是，在每个生命阶段表达水平最高的基因中，都有绦虫特异性疏水性配体结合蛋白（HLBP）家族的不同成员。我们还发现了从囊尾蚴到成虫转变过程中主要代谢变化的转录组特征。因此，这项工作有助于揭示伴随这种重要绦虫模型物种发育的基因表达变化，据我们所知，这是首次对涵盖绦虫主要生命阶段的所有阶段进行稳健重复的转录组研究。
