Basika T, Paludo G P, Araujo F M, Salim A C, Pais F, Maldonado L, Macchiaroli N, Camargo de Lima J, Rosenzvit M, Oliveira G C, Kamenetzky L, Ferreira H B
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Laboratório de Biologia Molecular de Cestódeos, CBiot, UFRGS, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Biologia Celular e Molecular, CBiot, UFRGS, Porto Alegre, RS, Brazil.
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Biologia Celular e Molecular, CBiot, UFRGS, Porto Alegre, RS, Brazil.
Mol Biochem Parasitol. 2019 Apr;229:35-46. doi: 10.1016/j.molbiopara.2019.02.006. Epub 2019 Feb 21.
Cestode development involves complex morphological and physiological changes. Here, we performed a differential expression analysis of gene transcripts between two developmental stages of the model cestode Mesocestoides corti. A RNA-seq-based approach was used to compare the transcriptomes of the tetrathyridium (larval, TT) and strobilated worm (ST) stages of the parasite. We found 19,053 transcripts, from which ∼45% were complete matches to genes previously annotated in the available M. corti draft genome sequence, ∼24% were considered novel isoforms, and ∼24% were considered potential novel transcripts. Stage-specific transcripts were found for both TTs (66) and STs (136), along with shared transcripts significantly overrepresented in one stage (342 in TTs, and 559 in STs). Differential expression and Gene Ontology term enrichment analyzes provided evidence of upregulation of different sets of transcripts associated with 'cytoskeleton', 'metabolism' and 'oxidation-reduction' processes in each stage, suggesting functional involvement of the corresponding genes with stage-specific features. Transcripts and processes enriched in the TT reflect typical larval processes that occur with the parasite in the intermediate host, such as asexual reproduction and budding, as well as active migration from the peritoneum to the liver and vice versa. In STs, transcripts associated with 'development', 'cell growth', and 'morphogenesis' were enriched, along with processes related to sexual reproduction, represented by the upregulation of numerous transcription factors, protein kinases, and histones. Overall, our results contributed to significantly increase the knowledge on the M. corti gene repertoire and expression profile in two developmental stages. Functional implications for the biology of larval and adult cestode parasites and for host-parasite interactions are discussed.
绦虫的发育涉及复杂的形态和生理变化。在此，我们对模式绦虫中殖孔绦虫（Mesocestoides corti）两个发育阶段的基因转录本进行了差异表达分析。采用基于RNA测序的方法比较了该寄生虫的四盘蚴（幼虫期，TT）和成虫期（ST）阶段的转录组。我们发现了19,053个转录本，其中约45%与先前在可用的中殖孔绦虫基因组草图序列中注释的基因完全匹配，约24%被认为是新的异构体，约24%被认为是潜在的新转录本。在TT（66个）和ST（136个）阶段均发现了阶段特异性转录本，同时还发现了在一个阶段中显著富集的共享转录本（TT中有342个，ST中有559个）。差异表达和基因本体术语富集分析提供了证据，表明每个阶段中与“细胞骨架”、“代谢”和“氧化还原”过程相关的不同转录本集上调，这表明相应基因参与了具有阶段特异性特征的功能。TT中富集的转录本和过程反映了寄生虫在中间宿主体内发生的典型幼虫过程，如无性繁殖和出芽，以及从腹膜到肝脏的活跃迁移，反之亦然。在ST中，与“发育”、“细胞生长”和“形态发生”相关的转录本富集，同时还有与有性繁殖相关的过程，表现为众多转录因子、蛋白激酶和组蛋白的上调。总体而言，我们的结果显著增加了对中殖孔绦虫在两个发育阶段的基因库和表达谱的认识。讨论了幼虫和成虫绦虫寄生虫生物学以及宿主 - 寄生虫相互作用的功能意义。
