采用 RT-PCR 快速检测 SARS-CoV-2，包括复制型和非复制型。
Rapid detection of SARS-CoV-2, replicating or non-replicating, using RT-PCR.
机构信息
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Haid Research Institute, Guangdong HaidGroup Co., Ltd., Guangzhou, China.
出版信息
Int J Infect Dis. 2021 Mar;104:471-473. doi: 10.1016/j.ijid.2021.01.043. Epub 2021 Jan 21.
To identify animals susceptible to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection or to determine whether SARS-CoV-2 contaminated meat is from a SARS-CoV-2-infected animal, a convenient and safe method was developed for rapid detection of SARS-CoV-2 in a replicating or non-replicating status in samples using reverse transcriptase-polymerase chain reaction (RT-PCR). This strategy can also be applied to develop assays for the detection of other viruses, either replicating or not.
为了鉴定易感染严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的动物，或者确定受 SARS-CoV-2 污染的肉类是否来自感染 SARS-CoV-2 的动物，本研究开发了一种简便、安全的方法，利用逆转录-聚合酶链反应（RT-PCR），快速检测样本中处于复制或非复制状态的 SARS-CoV-2。该策略还可用于开发检测其他病毒的检测方法，无论这些病毒是否具有复制能力。
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