用于检测复制 SARS-CoV-2 的特异性反转录 PCR
Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2.
出版信息
Emerg Infect Dis. 2021 Feb;27(2):632-635. doi: 10.3201/eid2702.204168.
Abstract
We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
摘要
我们开发了一种检测负链 RNA 的检测方法，作为严重急性呼吸综合征冠状病毒 2 （SARS-CoV-2）正在复制的替代指标。我们在症状出现后长达 30 天的 41 名冠状病毒疾病患者中检测到了负链 RNA。该检测方法可能为临床判断患者的传染性提供依据。
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