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用于检测复制 SARS-CoV-2 的特异性反转录 PCR

Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2.

出版信息

Emerg Infect Dis. 2021 Feb;27(2):632-635. doi: 10.3201/eid2702.204168.

DOI:10.3201/eid2702.204168
PMID:33496233
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7853532/
Abstract

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.

摘要

我们开发了一种检测负链 RNA 的检测方法，作为严重急性呼吸综合征冠状病毒 2 （SARS-CoV-2）正在复制的替代指标。我们在症状出现后长达 30 天的 41 名冠状病毒疾病患者中检测到了负链 RNA。该检测方法可能为临床判断患者的传染性提供依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/7853532/a958faf1edad/20-4168-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/7853532/f23db10cce4a/20-4168-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/7853532/a958faf1edad/20-4168-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/7853532/f23db10cce4a/20-4168-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4a6/7853532/a958faf1edad/20-4168-F2.jpg

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Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2.

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Testing for SARS-CoV-2: lessons learned and current use cases.

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[2]
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[3]
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Infect Control Hosp Epidemiol. 2024-2

[4]
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EBioMedicine. 2023-7

[5]
Viral Clearance with Neutrophil Recovery in a Patient with Active COVID-19 Infection and Refractory Acute Myeloid Leukemia Who Underwent Successful Reinduction with Cytarabine/Idarubicin.

Case Rep Oncol. 2022-8-3

[6]
Considerations regarding Interpretation of Positive SARS-CoV-2 Molecular Results with Late Cycle Threshold Values.

J Clin Microbiol. 2022-9-21

[7]
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[8]
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[10]
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本文引用的文献

[1]
SARS-CoV-2 Virus Culture and Subgenomic RNA for Respiratory Specimens from Patients with Mild Coronavirus Disease.

Emerg Infect Dis. 2020-8-4

[2]
Viable SARS-CoV-2 in various specimens from COVID-19 patients.

Clin Microbiol Infect. 2020-7-23

[3]
Persistent detection of SARS-CoV-2 RNA in patients and healthcare workers with COVID-19.

J Clin Virol. 2020-5-30

[4]
Predicting Infectious Severe Acute Respiratory Syndrome Coronavirus 2 From Diagnostic Samples.

Clin Infect Dis. 2020-12-17

[5]
To Interpret the SARS-CoV-2 Test, Consider the Cycle Threshold Value.

Clin Infect Dis. 2020-11-19

[6]
Prolonged Persistence of SARS-CoV-2 RNA in Body Fluids.

Emerg Infect Dis. 2020-5-8

[7]
Comparison of the Panther Fusion and a laboratory-developed test targeting the envelope gene for detection of SARS-CoV-2.

J Clin Virol. 2020-4-24

[8]
Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards.

Eur J Clin Microbiol Infect Dis. 2020-4-27

[9]
Virological assessment of hospitalized patients with COVID-2019.

Nature. 2020-4-1

[10]
Subgenomic messenger RNAs: mastering regulation of (+)-strand RNA virus life cycle.

Virology. 2011-3-5

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