Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
Sci Total Environ. 2021 May 15;769:145219. doi: 10.1016/j.scitotenv.2021.145219. Epub 2021 Jan 18.
An ex situ fermentation system (EFS) can efficiently transform and utilize nitrogen in swine wastewater and reduce environmental pollution. High-throughput sequencing was used to study the relationship between the succession of total bacteria, fungi, and functional bacteria in a swine wastewater EFS, as well as nitrogen metabolism and environmental factors. During the fermentation process, inorganic nitrogen gradually accumulated and the pH changed rapidly from weakly acidic to alkaline. The dominant genera of bacteria, fungi and functional bacteria carrying amoA, nirK, and nosZ genes changed gradually, and Clostridium sensu stricto 1, Thermomyces, Nitrosomonas, Mesorhizobium, and Pseudomonas genera became the most abundant, which showed positive correlations with temperature, pH, and nitrogen levels. Other changed populations showed different correlations with environmental factors, and physical-chemical factors explained more variation of microorganisms than nitrogen resources. These findings contribute to a comprehensive understanding of nitrogen metabolism in EFSs from a molecular micro-ecology perspective.
一种异位发酵系统（EFS）可以有效地转化和利用猪废水中的氮，减少环境污染。高通量测序用于研究猪废水 EFS 中总细菌、真菌和功能细菌的演替以及氮代谢与环境因素之间的关系。在发酵过程中，无机氮逐渐积累，pH 值从弱酸性迅速变为碱性。携带 amoA、nirK 和 nosZ 基因的细菌、真菌和功能细菌的优势属逐渐发生变化，Clostridium sensu stricto 1、Thermomyces、Nitrosomonas、Mesorhizobium 和 Pseudomonas 属成为最丰富的属，它们与温度、pH 值和氮水平呈正相关。其他变化的种群与环境因素表现出不同的相关性，理化因素对微生物的解释变异大于氮源。这些发现有助于从分子微观生态学的角度全面了解 EFS 中的氮代谢。
