College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences (IEDA, CAAS), Beijing, China.
Bioresour Technol. 2019 Jul;284:266-275. doi: 10.1016/j.biortech.2019.03.135. Epub 2019 Mar 28.
The ectopic fermentation system (EFS) is an advanced technology for treating farm wastewater, and it reduces ammonia nitrogen emission and nitrogen loss of fermentation products. This study observed the functional bacteria succession related to nitrogen metabolism in EFS by high throughput sequencing, and evaluated their associations with environmental factors. Results revealed that with the changes of temperature, pH, moisture content, and nitrogen content during fermentation, the species richness and diversity of ammonia oxidizing bacteria (AOB) with amoA increased, but those of denitrifying bacteria carrying nirK and nosZ decreased. During the fermentation process, the dominant bacterial populations of AOB and denitrifying bacteria changed significantly, and different bacterial populations showed different positive/negative correlations with the environmental factors. This study revealed the role of functional bacteria in ammonia removal and nitrogen conservation of EFS, and provided a theoretical basis for the improvement of microbial agents and EFS application.
异位发酵系统（EFS）是一种处理农业废水的先进技术，它可以减少氨氮排放和发酵产物的氮损失。本研究通过高通量测序观察了 EFS 中与氮代谢相关的功能细菌演替，并评估了它们与环境因素的关系。结果表明，随着发酵过程中温度、pH 值、含水量和氮含量的变化，amoA 基因丰度增加的氨氧化细菌（AOB）的物种丰富度和多样性增加，而携带 nirK 和 nosZ 的反硝化细菌的物种丰富度和多样性则降低。在发酵过程中，AOB 和反硝化细菌的优势菌群发生了显著变化，不同菌群与环境因素表现出不同的正/负相关性。本研究揭示了功能细菌在 EFS 氨去除和氮保存中的作用，为微生物制剂和 EFS 应用的改进提供了理论依据。
