Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Methods Mol Biol. 2021;2241:193-198. doi: 10.1007/978-1-0716-1095-4_16.
The process of extracellular DNA trap release by leukocytes, including eosinophils, has been considered as an important cell-mediated immune response to different inflammatory stimuli helping to understand the physiopathology of many diseases. Here we describe in detail two useful and simple protocols for a semiquantitative and a qualitative analysis of extracellular DNA traps released by human eosinophils, based on fluorimetry and fluorescence microscopy, respectively. These methods can also be applied to detect the DNA trap release by other leukocytes such as neutrophils and even other cell types.
白细胞（包括嗜酸性粒细胞）释放细胞外 DNA 陷阱的过程被认为是对不同炎症刺激的重要细胞介导免疫反应，有助于理解许多疾病的病理生理学。在这里，我们详细描述了两种基于荧光法和荧光显微镜的分别用于半定量和定性分析人嗜酸性粒细胞释放的细胞外 DNA 陷阱的有用且简单的方案。这些方法也可应用于检测其他白细胞（如中性粒细胞）甚至其他细胞类型释放的 DNA 陷阱。
