Primary Industries Development, Department of Primary Industries and Regional Development, Kensington, Western Australia 6151, Australia.
Sustainability and Biosecurity, Department of Primary Industries and Regional Development, Kensington, Western Australia 6151, Australia.
Plant Dis. 2021 Sep;105(9):2484-2493. doi: 10.1094/PDIS-10-20-2312-RE. Epub 2021 Oct 1.
Turnip yellows virus (TuYV; family , genus ) is the most widespread and economically damaging virus of canola ( L.) production in Australia. However, no Australian commercial seed companies market TuYV-resistant canola cultivars, and little information is available on the susceptibility of those available. To identify potential sources of TuYV resistance, 100 accessions from the ERANET ASSYST diversity set were screened in the field and five of these were selected for further phenotyping via aphid inoculation. Furthermore, 43 Australian canola cultivars, six genotypes with previously reported resistance, and 33 and cultivars were phenotyped. All Australian cultivars were susceptible except for 'ATR Stingray'. Stronger resistance to systemic TuYV infection (IR) was identified in diversity set accessions 'Liraspa-A', 'SWU Chinese 3', and 'SWU Chinese 5'. As indicated by lower relative enzyme-linked immunosorbent assay absorbance values (-) in infected plants, resistance to TuYV accumulation (AR) often accompanied IR. Moderate IR was identified in four cultivars and one cultivar. Very strong AR was identified in four cultivars and AR of some degree was common across many cultivars of this species tested. The impact of temperature during the inoculation access period or post-inoculation incubation on the resistance identified was examined. Infection rates were significantly higher in resistant genotypes when inoculated at 16°C than at 26°C, suggesting an increase in aphid transmission efficiency. IR in genotypes was strong when incubated at 16°C, but weakened at elevated temperatures with almost total breakdown in most genotypes at 30°C. However, infected plants of and genotypes with AR maintained lower - values than susceptible controls at all temperatures tested. Novel sources of resistance identified in this study offer potential as breeding material in Australia and abroad.
芜菁黄花叶病毒（TuYV；家族 ，属）是澳大利亚油菜（油菜）生产中分布最广泛、经济危害最大的病毒。然而，没有澳大利亚商业种子公司销售 TuYV 抗性油菜品种，可用品种的敏感性信息也很少。为了鉴定 TuYV 抗性的潜在来源，从 ERANET ASSYST 多样性组中筛选了 100 个品系进行田间筛选，其中 5 个品系通过蚜虫接种进行了进一步的表型分析。此外，还对 43 个澳大利亚油菜品种、6 个具有先前报道抗性的基因型以及 33 个 和 品种进行了表型分析。除了“ATR Stingray”外，所有澳大利亚品种均易感。在多样性组中，品系“Liraspa-A”、“SWU Chinese 3”和“SWU Chinese 5”对系统性 TuYV 感染（IR）的抗性更强。在感染植株中相对酶联免疫吸附测定（ELISA）吸光度值（-）较低，表明对 TuYV 积累（AR）的抗性通常伴随着 IR。在四个 品种和一个 品种中鉴定出中度 IR。在四个 品种和一个 品种中鉴定出很强的 IR，在该种许多测试品种中普遍存在某种程度的 AR。在接种时期或接种后孵育期间温度对鉴定出的抗性的影响进行了检验。在 16°C 下接种时，抗性基因型的感染率明显高于 26°C，表明蚜虫传播效率提高。在 16°C 下， 基因型的 IR 很强，但在高温下减弱，大多数基因型在 30°C 下几乎完全失效。然而，具有 AR 的 和 基因型的感染植株在所有测试温度下的 - 值均低于敏感对照。本研究中鉴定出的新型抗性来源为澳大利亚和国外的育种材料提供了潜力。
