Industry and Economic Development, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, Kensington, Western Australia, 6151, Australia.
Industry and Economic Development, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, Kensington, Western Australia, 6151, Australia.
Virus Res. 2020 Feb;277:197847. doi: 10.1016/j.virusres.2019.197847. Epub 2019 Dec 27.
Turnip yellows virus (TuYV; family Luteoviridae, genus Polerovirus) is the most economically damaging virus infecting canola (Brassica napus) in the south-west Australian grainbelt. However, the impact of TuYV infection at different growth stages on canola seed yield has not been examined. This information is vital for implementing targeted management strategies. Four glasshouse experiments were conducted to examine seed yield losses incurred by an open-pollinated (ATR Bonito) and hybrid (Hyola® 404RR) canola cultivar when aphid-inoculated with TuYV at GS12 (two leaves unfolded), GS17 (seven leaves unfolded), GS30 (beginning of stem elongation) and GS65 (full flowering). When inoculated at GS12 and GS17, cv. Bonito plants incurred 30 % and 36 % seed yield losses, respectively, compared to healthy plants. Similarly, cv. 404RR incurred 41 % and 26 % seed yield losses at GS12 and GS17, respectively. However, when inoculated at GS30, whilst cv. Bonito plants incurred a 26 % seed yield loss, cv. 404RR incurred no significant loss. Neither cultivar incurred seed yield losses from inoculation at GS65. Additional information was collected from these experiments to improve sampling protocols to enhance TuYV detection, with a molecular and serological technique. When canola plants were at pre-flowering growth stages, TuYV was reliably detected 7-14 days after inoculation (DAI) in the youngest leaf. Once flowering had begun, TuYV was consistently detected 7-14 DAI in petals and flower buds. In contrast, regardless of growth stage, testing the oldest leaf regularly resulted in delayed detection or false negatives. Information generated in this study helps to quantify the value of management strategies targeted at preventing TuYV spread in pre-flowering canola crops and ultimately increase the efficiency of resource use.
芜菁黄花叶病毒（TuYV；家族 Luteoviridae，属 Polerovirus）是西南澳大利亚谷物带感染油菜（Brassica napus）的最具经济破坏性的病毒。然而，TuYV 感染对油菜种子产量的影响在不同的生长阶段尚未被研究。这一信息对于实施有针对性的管理策略至关重要。进行了四项温室实验，以研究在 GS12（两叶展开）、GS17（七叶展开）、GS30（茎开始伸长）和 GS65（盛花期）时，用桃蚜接种感病的油菜（ATR Bonito）和杂交油菜（Hyola® 404RR）后种子产量的损失。在 GS12 和 GS17 接种时，Bonito 品种的种子产量分别损失了 30%和 36%，而健康植株则没有损失。类似地，404RR 品种的种子产量分别损失了 41%和 26%。然而，在 GS30 接种时，Bonito 品种的种子产量损失了 26%，而 404RR 品种则没有显著损失。在 GS65 接种时，两个品种都没有出现种子产量损失。从这些实验中收集了更多信息，以改进采样方案，使用分子和血清学技术来提高 TuYV 的检测率。在油菜处于开花前的生长阶段时，在接种后 7-14 天（DAI），在最年轻的叶片中可靠地检测到 TuYV。一旦开始开花，TuYV 在花瓣和花蕾中始终在 7-14 DAI 时被检测到。相比之下，无论生长阶段如何，定期检测最老的叶片通常会导致延迟检测或假阴性。本研究提供的信息有助于量化针对防止 TuYV 在开花前油菜作物中传播的管理策略的价值，并最终提高资源利用效率。
