Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.
Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Nat Prod Res. 2022 Mar;36(5):1391-1395. doi: 10.1080/14786419.2021.1876046. Epub 2021 Jan 25.
The current biologically guided study aimed the investigation of cytotoxic activity, identification of the phytochemical content of aerial parts and supporting this activity by a molecular docking study. Aqueous fraction demonstrated the most potent cytotoxic effect against CACO-2 with IC = 0.067 ± 0.01 μg/mL. Furthermore, EtOAc fraction showed a remarkable cytotoxic activity against MCF-7 cell line with IC = 0.35 ± 0.02 μg/mL. Consequently, total ethanolic extract (TEE) and its fractions were subjected to LC-HR-ESI-MS metabolic profiling to discover the constituents that possibly underlie their cytotoxicity. Twenty compounds were tentatively identified from metabolic analysis. Furthermore, eight compounds were isolated. docking study revealed that stachydrine is more likely to account for the antiproliferative activity of both EtOAc and aqueous fractions, probably its moderate inhibition of receptor tyrosine kinases. [Formula: see text].
当前的生物导向研究旨在研究细胞毒性活性、鉴定地上部分的植物化学成分，并通过分子对接研究支持这种活性。水相部分对 CACO-2 表现出最强的细胞毒性作用，IC = 0.067 ± 0.01 μg/mL。此外，EtOAc 部分对 MCF-7 细胞系表现出显著的细胞毒性活性，IC = 0.35 ± 0.02 μg/mL。因此，总醇提物（TEE）及其馏分进行了 LC-HR-ESI-MS 代谢物分析，以发现可能导致其细胞毒性的成分。从代谢分析中暂定鉴定了 20 种化合物。此外，还分离了 8 种化合物。对接研究表明，水苏碱可能是 EtOAc 和水相部分的抗增殖活性的原因，可能是其对受体酪氨酸激酶的中等抑制作用。[公式：见文本]。
