Bezmialem Vakif University, Faculty of Pharmacy, Department of Pharmaceutical Botany, 34093, Fatih-Istanbul, Turkey.
Bezmialem Vakif University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 34093, Fatih-Istanbul, Turkey; Bezmialem Vakif University, Drug Application and Research Center, 34093, Istanbul, Turkey.
J Ethnopharmacol. 2021 Aug 10;276:114211. doi: 10.1016/j.jep.2021.114211. Epub 2021 May 18.
Dittrichia viscosa (L.) Greuter ("Sarı ot, Yapışkan andız otu" in Turkish) is a medicinal plant that has been traditionally used in the Mediterranean area. This plant is used by the local population for the treatment of cancer. Investigation of their biological activities is therefore very important to be supported by scientific basis for traditional use.
In this study, it is aimed to assess the phytochemical composition, in vitro antioxidant, cytotoxic, and antiproliferative activities of the aqueous and ethanolic extracts obtained from the aerial parts (stems, leaves, flowers) of D. viscosa, collected from two sites in Turkey (Istanbul and Marmaris) against breast and prostate tumor cell lines.
Validated methods were used to evaluate the in vitro antioxidant capacity (DPPH, ABTS, CUPRAC), cytotoxicity (Cell Viability Assay), antiproliferative (Apoptosis assay), and phytochemical compositions. The nepetin (N), 3-O-methylquercetin (Q), and hispidulin (H) in the extracts of D. viscosa were quantified by HPLC and LC-HRMS. Furthermore, in order to control the standards of benefiting from the plant in a healthy way, the contents of some heavy metals were also assessed by ICP-OES in the plant and soil samples as well as the species soil's physical and chemical characteristics.
We have found that heavy metal accumulation in the soil does not exceed the allowable limit value except for the nickel. The results showed that ethanol extraction is an efficient strategy to get NQH molecules with a higher content compared with other extraction techniques. However, using the same extraction method revealed that the amount of NQH molecules in the samples of two different regions were variable. The results suggested that all extracts had a high amount of total phenolic content (12.354-22.184 μg GAE/mg) and total flavonoid content (4.442-17.263 μg QE/g). In the antioxidant assay according to the DPPH method, the aqueous ethanol extracts (IC; 21.00 μg/mL) showed stronger antioxidant activity than BHT. A significant reduction in cell viability was particularly observed in MDA-MB-231 cells, which were sensitive to ethanolic extracts in Istanbul (12-22%) and in Marmaris (14-15%), while PC3 cell lines were also more sensitive to extracts of the aqueous in Istanbul (16%) and the decoction in Marmaris (12%) after 72 h. Especially, it was observed that Marmaris and Istanbul samples induced the toxicity against PC3 cells.
The study supports the medicinal use of D. viscosa as a potential anticancer against breast and prostate cancer cells in vitro and underlines the immense therapeutic potential of the plant.
Dittrichia viscosa（L.）Greuter（土耳其语中的“Sarı ot，Yapışkan andız otu”）是一种药用植物，传统上在地中海地区使用。当地居民用这种植物治疗癌症。因此，为了支持传统用途，研究其生物活性非常重要。
本研究旨在评估从 D. viscosa 的地上部分（茎、叶、花）获得的水提物和醇提物的植物化学成分、体外抗氧化、细胞毒性和抗增殖活性，该植物分别来自土耳其的两个地点（伊斯坦布尔和马尔马里斯），用于乳腺癌和前列腺肿瘤细胞系。
采用经过验证的方法评估体外抗氧化能力（DPPH、ABTS、CUPRAC）、细胞毒性（细胞活力测定）、抗增殖（细胞凋亡测定）和植物化学成分。通过 HPLC 和 LC-HRMS 定量测定 D. viscosa 提取物中的 Nepetin（N）、3-O-甲基槲皮素（Q）和毛蕊花糖苷（H）。此外，为了控制以健康方式从植物中获益的标准，还通过 ICP-OES 评估了植物和土壤样本以及物种土壤的物理和化学特性中的一些重金属含量。
我们发现，除镍外，土壤中的重金属积累并未超过允许限值。结果表明，与其他提取技术相比，乙醇提取是获得含有更高含量 NQH 分子的有效策略。然而，使用相同的提取方法表明，来自两个不同地区的样本中 NQH 分子的数量是可变的。结果表明，所有提取物均具有高含量的总酚含量（12.354-22.184μgGAE/mg）和总类黄酮含量（4.442-17.263μgQE/g）。在根据 DPPH 法进行的抗氧化测定中，水乙醇提取物（IC；21.00μg/mL）表现出比 BHT 更强的抗氧化活性。在 MDA-MB-231 细胞中观察到细胞活力显著降低，伊斯坦布尔（12-22%）和马尔马里斯（14-15%）的乙醇提取物对其敏感，而 PC3 细胞系在伊斯坦布尔（16%）和马尔马里斯（12%）的水提物和煎剂 72 小时后也对提取物更敏感。特别是，观察到马尔马里斯和伊斯坦布尔的样本对 PC3 细胞产生了毒性。
该研究支持将 D. viscosa 作为一种潜在的抗癌药物用于体外乳腺癌和前列腺癌细胞，并强调了该植物的巨大治疗潜力。
