• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种用于抗胃食管反流病医疗器械初步功能评估的新型酸诱导猪食管损伤模型。

A novel porcine model of acid-induced esophageal damage for preliminary functional evaluations of anti-gastroesophageal reflux disease medical devices.

作者信息

Ventrella Domenico, Salaroli Roberta, Elmi Alberto, Carnevali Giacomo, Forni Monica, Baldi Fabio, Bacci Maria Laura

机构信息

Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO), Italy.

Center for the Study of Diseases of the Esophagus, University of Bologna, Bologna, Italy.

出版信息

Vet World. 2020 Dec;13(12):2728-2735. doi: 10.14202/vetworld.2020.2728-2735. Epub 2020 Dec 21.

DOI:10.14202/vetworld.2020.2728-2735
PMID:33487991
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7811544/
Abstract

AIM

The aim of the study was to set up a porcine model of acid-induced damage and to evaluate its performance by means of multichannel intraluminal impedance and pH (MII-pH) live recording, histology, and Evans blue (EB) permeability assay.

MATERIALS AND METHODS

Thirteen esophagi, collected at a slaughterhouse, were ablated of their sphincters, pinned upright on a support, and placed in a thermostatic hood at 37°C with two infusion tubes and an MII-pH probe inserted in the top end. Three esophagi (histology controls) were only left in the hood for 3.5 h before sampling, while the remaining organs underwent the experimental protocol including saline infusion and recovery recording, and acid solution infusion and recovery recording.

RESULTS

MII-pH analysis highlighted a significantly stronger decrease during acid infusion when compared to saline, but a better post-infusion recovery for saline solution. At the end of the protocol, MII was still statistically lower than baseline. The acid-damaged esophagi significantly absorbed more EB dye, and histology revealed strong mucosal exfoliation.

CONCLUSION

The proposed model of esophageal acid damage seems to be repeatable, reliable, and achievable using organs collected at the slaughterhouse. MII recording proved to have good sensitivity in detecting mucosal alterations also in trials.

摘要

目的

本研究的目的是建立一个酸诱导损伤的猪模型，并通过多通道腔内阻抗和pH值（MII-pH）实时记录、组织学检查和伊文思蓝（EB）通透性测定来评估其性能。

材料与方法

从屠宰场收集13个食管，切除其括约肌，垂直固定在支架上，置于37°C的恒温箱中，在顶端插入两根输液管和一个MII-pH探头。三个食管（组织学对照）仅在恒温箱中放置3.5小时后取样，其余器官进行实验方案，包括生理盐水输注和恢复记录，以及酸溶液输注和恢复记录。

结果

MII-pH分析显示，与生理盐水相比，酸输注期间下降明显更强烈，但生理盐水溶液输注后恢复更好。在实验方案结束时，MII在统计学上仍低于基线。酸损伤的食管显著吸收更多的EB染料，组织学检查显示黏膜有强烈的剥脱。

结论

所提出的食管酸损伤模型似乎是可重复的、可靠的，并且使用在屠宰场收集的器官即可实现。MII记录在试验中也被证明在检测黏膜改变方面具有良好的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/4db0373a7c18/Vetworld-13-2728-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/8e93fba267ad/Vetworld-13-2728-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/67ebbaa59b9f/Vetworld-13-2728-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/d3ef7792812c/Vetworld-13-2728-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/5b26440114d8/Vetworld-13-2728-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/e26b1143529a/Vetworld-13-2728-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/4db0373a7c18/Vetworld-13-2728-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/8e93fba267ad/Vetworld-13-2728-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/67ebbaa59b9f/Vetworld-13-2728-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/d3ef7792812c/Vetworld-13-2728-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/5b26440114d8/Vetworld-13-2728-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/e26b1143529a/Vetworld-13-2728-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e9/7811544/4db0373a7c18/Vetworld-13-2728-g006.jpg

相似文献

1
A novel porcine model of acid-induced esophageal damage for preliminary functional evaluations of anti-gastroesophageal reflux disease medical devices.一种用于抗胃食管反流病医疗器械初步功能评估的新型酸诱导猪食管损伤模型。
Vet World. 2020 Dec;13(12):2728-2735. doi: 10.14202/vetworld.2020.2728-2735. Epub 2020 Dec 21.
2
Comparison of multichannel intraluminal impedance-pH monitoring and reflux scintigraphy in pediatric patients with suspected gastroesophageal reflux.多通道腔内阻抗-pH监测与反流闪烁显像在疑似胃食管反流儿科患者中的比较
World J Gastroenterol. 2016 Nov 21;22(43):9595-9603. doi: 10.3748/wjg.v22.i43.9595.
3
Swallow evaluation during multichannel intraluminal impedance and pH: an alternate method to assess esophageal transit.多通道腔内阻抗和 pH 值监测期间的吞咽评估：评估食管传输的替代方法。
J Clin Gastroenterol. 2011 Nov-Dec;45(10):862-6. doi: 10.1097/MCG.0b013e31822a2c61.
4
Combined esophageal multichannel intraluminal impedance and pH monitoring (MII -pH) in the diagnostics and treatment of gastroesophageal reflux disease and its complications.联合食管多通道腔内阻抗与pH监测（MII -pH）在胃食管反流病及其并发症的诊断和治疗中的应用
Pol Przegl Chir. 2011 Sep;83(9):488-96. doi: 10.2478/v10035-011-0076-7.
5
Can multichannel intraluminal pH-impedance monitoring be limited to 3 hours? Comparison between ambulatory 24-hour and post-prandial 3-hour recording.多通道腔内pH值-阻抗监测能否限制在3小时？动态24小时记录与餐后3小时记录的比较。
Dis Esophagus. 2014 Nov-Dec;27(8):732-6. doi: 10.1111/dote.12161. Epub 2013 Nov 20.
6
Small volume acid reflux in gastroesophageal reflux disease patients with hiatal hernia is only detectable by pH-metry but not by multichannel intraluminal impedance.对于合并食管裂孔疝的胃食管反流病患者，小容量酸反流仅能通过 pH 监测而不是多通道腔内阻抗技术检测到。
Dis Esophagus. 2013 Jul;26(5):544-8. doi: 10.1111/j.1442-2050.2012.01379.x. Epub 2012 Aug 2.
7
Characterization of patients with low baseline impedance on multichannel intraluminal impedance-pH reflux testing.多通道腔内阻抗-pH 反流测试中基线阻抗低的患者特征。
J Clin Gastroenterol. 2012 Aug;46(7):e55-7. doi: 10.1097/MCG.0b013e318247c319.
8
The sensitivity of multichannel intraluminal impedance and the pH probe in the evaluation of gastroesophageal reflux in children.多通道腔内阻抗和pH探头在评估儿童胃食管反流中的敏感性。
Clin Gastroenterol Hepatol. 2006 Feb;4(2):167-72. doi: 10.1016/s1542-3565(05)00854-2.
9
Combined multichannel intraluminal impedance and pH esophageal testing compared to pH alone for diagnosing both acid and weakly acidic gastroesophageal reflux.联合多通道腔内阻抗和pH值食管检测与单纯pH值检测在诊断酸性和弱酸性胃食管反流方面的比较。
Clin Gastroenterol Hepatol. 2007 Feb;5(2):172-7. doi: 10.1016/j.cgh.2006.11.015.
10
Laparoscopic Nissen fundoplication mainly reduces the volume of acid reflux and potentially improves mucosal integrity up to the middle esophagus in neurologically impaired children detected by esophageal combined pH-multichannel intraluminal impedance measurements.通过食管联合pH值-多通道腔内阻抗测量发现，腹腔镜下Nissen胃底折叠术主要减少神经功能受损儿童的胃酸反流体积，并可能改善直至食管中段的黏膜完整性。
J Pediatr Surg. 2016 Aug;51(8):1283-7. doi: 10.1016/j.jpedsurg.2016.01.012. Epub 2016 Feb 21.

引用本文的文献

1
Gelatin-Based Ingestible Impedance Sensor to Evaluate Gastrointestinal Epithelial Barriers.基于明胶的可食用阻抗传感器评估胃肠道上皮屏障。
Adv Mater. 2023 Apr;35(17):e2211581. doi: 10.1002/adma.202211581. Epub 2023 Mar 18.

本文引用的文献

1
Preliminary Assessment of the Mucosal Toxicity of Tea Tree () and Rosemary () Essential Oils on Novel Porcine Uterus Models.茶树（）和迷迭香（）精油对新型猪子宫模型的黏膜毒性的初步评估。
Int J Mol Sci. 2020 May 9;21(9):3350. doi: 10.3390/ijms21093350.
2
Lubiprostone protects esophageal mucosa from acid injury in porcine esophagus.鲁比前列酮可保护猪食管黏膜免受酸损伤。
Am J Physiol Gastrointest Liver Physiol. 2020 Apr 1;318(4):G613-G623. doi: 10.1152/ajpgi.00086.2019. Epub 2020 Feb 18.
3
Mucosal Impedance: a New Approach to Diagnosing Gastroesophageal Reflux Disease and Eosinophilic Esophagitis.
黏膜阻抗：诊断胃食管反流病和嗜酸性食管炎的新方法。
Curr Gastroenterol Rep. 2018 Jun 9;20(7):33. doi: 10.1007/s11894-018-0639-4.
4
The role of esophageal pH-impedance testing in clinical practice.食管pH-阻抗测试在临床实践中的作用。
Curr Opin Gastroenterol. 2018 Jul;34(4):249-257. doi: 10.1097/MOG.0000000000000441.
5
Evaluation of Esophageal Mucosal Integrity in Patients with Gastroesophageal Reflux Disease.胃食管反流病患者食管黏膜完整性的评估。
Digestion. 2018;97(1):31-37. doi: 10.1159/000484106. Epub 2018 Feb 1.
6
Ductular and proliferative response of esophageal submucosal glands in a porcine model of esophageal injury and repair.猪食管损伤与修复模型中食管黏膜下腺的导管和增殖反应
Am J Physiol Gastrointest Liver Physiol. 2017 Sep 1;313(3):G180-G191. doi: 10.1152/ajpgi.00036.2017. Epub 2017 Jun 1.
7
Chronic Cough Due to Gastroesophageal Reflux in Adults: CHEST Guideline and Expert Panel Report.成人胃食管反流所致慢性咳嗽：CHEST指南与专家小组报告
Chest. 2016 Dec;150(6):1341-1360. doi: 10.1016/j.chest.2016.08.1458. Epub 2016 Sep 7.
8
Protective effect of rhEPO on tight junctions of cerebral microvascular endothelial cells early following traumatic brain injury in rats.重组人促红细胞生成素对大鼠创伤性脑损伤后早期脑微血管内皮细胞紧密连接的保护作用。
Brain Inj. 2016;30(4):462-7. doi: 10.3109/02699052.2015.1080386. Epub 2016 Mar 2.
9
An Evidence-Based Approach to the Treatment of Gastroesophageal Reflux Disease.基于循证医学的胃食管反流病治疗方法。
JAMA Surg. 2016 Jan;151(1):73-8. doi: 10.1001/jamasurg.2015.4233.
10
An evaluation of the adhesion of solid oral dosage form coatings to the oesophagus.固体口服剂型包衣对食管黏附性的评价。
Int J Pharm. 2015 Dec 30;496(2):299-303. doi: 10.1016/j.ijpharm.2015.10.014. Epub 2015 Oct 21.