Guzmán M, Geelen M J
Laboratory of Veterinary Biochemistry, University of Utrecht, The Netherlands.
Biochem Biophys Res Commun. 1988 Mar 15;151(2):781-7. doi: 10.1016/s0006-291x(88)80349-8.
An assay procedure for carnitine palmitoyltransferase is described which allows rapid measurement of the overt activity of this enzyme in isolated rat hepatocytes. In a one-step procedure digitonin permeabilizes the plasma membrane and at the same time carnitine palmitoyltransferase activity is measured. The use of the present procedure shows that carnitine palmitoyltransferase activity is regulated on the short term by different types of agonists. Thus, insulin, epidermal growth factor, vasopressin and the phorbol ester PMA inhibit carnitine palmitoyltransferase activity, whereas glucagon treatment renders the enzyme more active. These changes in enzyme activity coincide with corresponding changes in the rate of fatty acid oxidation.
本文描述了一种肉碱棕榈酰转移酶的测定方法，该方法可快速测定分离的大鼠肝细胞中这种酶的明显活性。在一步法中，洋地黄皂苷可使质膜通透，同时测定肉碱棕榈酰转移酶的活性。使用本方法表明，肉碱棕榈酰转移酶的活性在短期内受到不同类型激动剂的调节。因此，胰岛素、表皮生长因子、血管加压素和佛波酯PMA可抑制肉碱棕榈酰转移酶的活性，而胰高血糖素处理则使该酶更具活性。酶活性这些变化与脂肪酸氧化速率的相应变化一致。
