Thumelin S, Esser V, Charvy D, Kolodziej M, Zammit V A, McGarry D, Girard J, Pegorier J P
Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement du CNRS, Meudon-Bellevue, France.
Biochem J. 1994 Jun 1;300 ( Pt 2)(Pt 2):583-7. doi: 10.1042/bj3000583.
The enzyme activity and the expression (protein and mRNA concentrations) of genes encoding for hepatic carnitine palmitoyl-transferases (CPT) I and II were studied during neonatal development, in response to nutritional state at weaning and during the fed-starved transition in adult rats. The activity, the protein concentration and the level of mRNA encoding CPT I are low in foetal-rat liver and increase 5-fold during the first day of extra-uterine life. The activity and gene expression of CPT I are high during the entire suckling period, in the liver of 30-day-old rats weaned at 20 days on to a high-fat diet and in the liver of 48 h-starved adult rats. The activity and CPT I gene expression are markedly decreased in the liver of rats weaned on to a high-carbohydrate diet. By contrast, the activity, the protein concentration and the level of mRNA encoding CPT II are already high in the liver of term foetuses and remain at this level throughout the suckling period, irrespective of the nutritional state of the animals either at weaning or in the adult.
在新生大鼠发育过程中、断奶时营养状态的响应以及成年大鼠进食-饥饿转换期间，研究了肝脏肉碱棕榈酰转移酶（CPT）I和II编码基因的酶活性及表达（蛋白质和mRNA浓度）。胎鼠肝脏中CPT I的活性、蛋白质浓度和mRNA水平较低，在宫外生活的第一天增加5倍。在整个哺乳期、20日龄断奶并喂食高脂饮食的30日龄大鼠肝脏以及饥饿48小时的成年大鼠肝脏中，CPT I的活性和基因表达都很高。在断奶后喂食高碳水化合物饮食的大鼠肝脏中，CPT I的活性和基因表达明显降低。相比之下，足月胎儿肝脏中CPT II的活性、蛋白质浓度和mRNA水平已经很高，并且在整个哺乳期都保持在这个水平，与动物在断奶时或成年后的营养状态无关。
