生物能量恢复现象的31P磁共振波谱研究

31P-MRS study of bio-energy recovering phenomenon.

作者信息

Yoshiyama M, Sakai H, Teragaki M, Takeuchi K, Takeda T, Ikata M, Ishikawa M, Miura I

机构信息

First Department of Internal Medicine, Osaka City University Medical School, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Mar 15;151(2):865-71. doi: 10.1016/s0006-291x(88)80361-9.

DOI:10.1016/s0006-291x(88)80361-9

PMID:3348817

Abstract

The ATP and creatine phosphate (PCr) contents in isolated guinea-pig hearts were determined by 31P-MRS measurement at 80.75 MHz using the Langendorff technique. Reperfusion of post-ischemic hearts with adenosine for 180 minutes increased ATP to 117.4% and decreased PCr to 59.8% of the preischemic value. Reperfusion without adenosine did not increase ATP and did not decrease PCr. The depressed cardiac function due to ischemia was remarkably improved in post-ischemic hearts by the increase in ATP due to adenosine. We found that the loss of ATP due to ischemia is not necessarily proportional to the extent of myocardial ischemic injury.

摘要

采用Langendorff技术，通过80.75MHz的31P磁共振波谱测量法测定了离体豚鼠心脏中的三磷酸腺苷（ATP）和磷酸肌酸（PCr）含量。缺血后心脏用腺苷再灌注180分钟，使ATP增加至缺血前值的117.4%，PCr降低至缺血前值的59.8%。无腺苷再灌注则不会增加ATP，也不会降低PCr。缺血后心脏中，腺苷使ATP增加，显著改善了因缺血导致的心脏功能抑制。我们发现，缺血导致的ATP损失不一定与心肌缺血损伤程度成正比。