雌二醇对水牛和山羊卵母细胞体外成熟的影响。
Effects of estradiol on in vitro maturation of buffalo and goat oocytes.
作者信息
Maksura Hurum, Akon Narsisa, Islam Md Nuronnabi, Akter Ireen, Modak Avijit Kumar, Khatun Asma, Alam Md Hasanur, Hashem Md Abul, Amin Md Ruhul, Moniruzzaman Mohammad
机构信息
Department of Animal Science Bangladesh Agricultural University Mymensingh Bangladesh.
出版信息
Reprod Med Biol. 2020 Sep 25;20(1):62-70. doi: 10.1002/rmb2.12350. eCollection 2021 Jan.
PURPOSE
The effects of estradiol on oocyte development seem to be varied among species. The present study investigated the effects of 17β-estradiol on in vitro maturation of buffalo and goat oocytes.
METHODS
Cumulus oocyte complexes (COCs) were aspirated from large antral follicles of slaughtered buffalo and goat ovaries. COCs were cultured in TCM-199 medium supplemented with 0, 0.5, 1, and 1.5 µg/mL of 17β-estradiol for in vitro maturation. Then, oocytes were used for the examination of state of nuclear maturation and cumulus expansion.
RESULTS
In both species, oocytes treated with 17β-estradiol showed higher cumulus expansion rate than control (0 µg/mL treated). In buffalo, the percentage of oocytes matured to the metaphase II (MII) stage increased in the concentration-dependent manner of 17β-estradiol. Similarly, estradiol positively influenced nuclear maturation of goat oocytes in vitro.
CONCLUSIONS
Estradiol has promoting effects on normalprogress of in vitro oocyte meiosis in buffalos and goats.
目的
雌二醇对卵母细胞发育的影响在不同物种间似乎存在差异。本研究调查了17β - 雌二醇对水牛和山羊卵母细胞体外成熟的影响。
方法
从屠宰的水牛和山羊卵巢的大型窦状卵泡中抽吸卵丘卵母细胞复合体（COCs）。将COCs在添加了0、0.5、1和1.5μg/mL 17β - 雌二醇的TCM - 199培养基中培养以进行体外成熟。然后，将卵母细胞用于核成熟状态和卵丘扩展的检查。
结果
在这两个物种中，用17β - 雌二醇处理的卵母细胞显示出比对照（0μg/mL处理）更高的卵丘扩展率。在水牛中，成熟到中期II（MII）期的卵母细胞百分比以17β - 雌二醇的浓度依赖性方式增加。同样，雌二醇在体外对山羊卵母细胞的核成熟有积极影响。
结论
雌二醇对水牛和山羊卵母细胞体外减数分裂的正常进程有促进作用。
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