Jiang Li, Li Yuning, Wang Fang, Zhang Xindao, Zhao Ruiping
Department of Pediatrics, The First Hospital of Lanzhou University, Lanzhou, China.
Dose Response. 2020 Dec 24;18(4):1559325820982189. doi: 10.1177/1559325820982189. eCollection 2020 Oct-Dec.
S-Allyl cysteine (SAC), an organic compound and a natural constituent of , commonly known as garlic have been consumed in routine foods are known to possess various biological activities. Nevertheless, scientific evidence on the protective effect of SAC against neonatal asthmatic rats is not available. Hence, the present study aimed at investigating the anti-asthmatic activity of SAC in neonatal asthmatic rats using Wistar rats. The study conducted in 4 groups consists of normal control rats, asthma-induced, asthma animals administered with SAC (25 mg/kg), and SAC control. At the end of the experimental period, inflammatory cells in bronchoalveolar lavage fluid (BALF), inflammatory markers, fibrinogen level, activated partial thromboplastin time, coagulation factor activity, and histopathology were elucidated. The current investigation exhibits that SAC significantly reduced the total leukocytes, with restored fibrinogen level, and activated partial thromboplastin time. In addition, the levels of inflammatory cytokines such as TNF-α (tumor necrosis factor- α), IL-6 (Interleukin 6), and IL-1β have also attenuated in SAC treated animals. Furthermore, the mRNA expression levels of (cyclooxygenase-2), (monocyte chemoattractant protein-1), regulated upon activation, normal T cell expressed and secreted), and were reduced in SAC treated animals. Treatment of rats with SAC significantly reduced inflammation and eosinophil infiltration in the lungs. These results suggest that SAC exert protection in neonatal asthmatic rats suffering from acute or chronic inflammation by inducing anti-inflammatory and cell-protective responses.
S-烯丙基半胱氨酸（SAC）是一种有机化合物，也是大蒜的天然成分，人们在日常食物中食用大蒜，已知其具有多种生物活性。然而，关于SAC对新生哮喘大鼠保护作用的科学证据尚不存在。因此，本研究旨在使用Wistar大鼠研究SAC对新生哮喘大鼠的抗哮喘活性。该研究分为4组，包括正常对照大鼠、哮喘诱导组、给予SAC（25mg/kg）的哮喘动物组和SAC对照组。在实验期结束时，对支气管肺泡灌洗液（BALF）中的炎性细胞、炎症标志物、纤维蛋白原水平、活化部分凝血活酶时间、凝血因子活性和组织病理学进行了阐明。目前的研究表明，SAC显著降低了总白细胞数量，恢复了纤维蛋白原水平和活化部分凝血活酶时间。此外，在接受SAC治疗的动物中，肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β等炎性细胞因子水平也有所降低。此外，在接受SAC治疗的动物中，环氧化酶-2（COX-2）、单核细胞趋化蛋白-1（MCP-1）、活化后正常T细胞表达和分泌的调节因子（RANTES）以及白细胞介素-4（IL-4）的mRNA表达水平均降低。用SAC治疗大鼠可显著减轻肺部炎症和嗜酸性粒细胞浸润。这些结果表明，SAC通过诱导抗炎和细胞保护反应，对患有急性或慢性炎症的新生哮喘大鼠发挥保护作用。
