Lee Jin-Seok, Kang Ji-Yun, Son Chang-Gue
Institute of Bioscience & Integrative Medicine, Dunsan Hospital of Daejeon University, Daejeon, South Korea.
Front Behav Neurosci. 2021 Jan 7;14:616389. doi: 10.3389/fnbeh.2020.616389. eCollection 2020.
This study aimed to help to understand the influence of stress on depression, which reflects the social environments of especially solitary life and the increasing prevalence of depressive disorders. To determine the distinguishable features of two-representative animal models of stress-induced depressive disorder, we compared isolation stress (IS) and unpredictable chronic mild stress (UCMS). After 4-week of stress, both models showed significant depressive- and anxiety-like behaviors in an open field test (OFT; < 0.01 for IS, < 0.01 for UCMS), forced swimming test (FST; < 0.01 for IS, < 0.01 for UCMS), and tail suspension test (TST; < 0.01 for IS, < 0.05 for UCMS) along with alterations in serum corticosterone levels, serotonin activity in the dorsal raphe nuclei (DRN) and microglial activity in the dentate gyrus of the hippocampus ( < 0.05 for both parameters). In a comparison of the two stress models, IS strongly induced depressive and anxiety features, as indicated by all parameters: behavior test scores ( < 0.05 for OFT, FST, and TST), serum corticosterone levels ( < 0.05), immunohistological alterations for serotonin activity ( < 0.05) and microglial activity ( = 0.072). Our results indicate the suitability of IS for the development of animal models of depressive disorders and may reveal the medical impact of social isolation environment in modern society.
本研究旨在帮助理解应激对抑郁症的影响，抑郁症反映了尤其是独居生活的社会环境以及抑郁症患病率的上升。为了确定应激诱导的抑郁症两种代表性动物模型的显著特征，我们比较了隔离应激（IS）和不可预测的慢性轻度应激（UCMS）。应激4周后，两种模型在旷场试验（OFT；IS组P<0.01，UCMS组P<0.01）、强迫游泳试验（FST；IS组P<0.01，UCMS组P<0.01）和悬尾试验（TST；IS组P<0.01，UCMS组P<0.05）中均表现出显著的抑郁样和焦虑样行为，同时血清皮质酮水平、中缝背核（DRN）中的5-羟色胺活性以及海马齿状回中的小胶质细胞活性也发生了改变（两个参数均P<0.05）。在两种应激模型的比较中，IS强烈诱导抑郁和焦虑特征，所有参数均表明：行为测试评分（OFT、FST和TST的P<0.05）、血清皮质酮水平（P<0.05）、5-羟色胺活性的免疫组织学改变（P<0.05）和小胶质细胞活性（P = 0.072）。我们的结果表明IS适用于开发抑郁症动物模型，并可能揭示现代社会中社会隔离环境的医学影响。
