School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China.
School of Nursing, Xinxiang Medical University, Xinxiang 453003, China.
Oxid Med Cell Longev. 2021 Jan 2;2021:7184760. doi: 10.1155/2021/7184760. eCollection 2021.
Recent studies have claimed that iron overload was correlated with the risk of hepatocellular carcinoma (HCC), and our previous studies have also demonstrated that dandelion polysaccharide (DP) suppressed HCC cell line proliferation via causing cell cycle arrest and inhibiting the PI3K/AKT/mTOR pathway, but the effect of DP on metabolism is still not very clear. Here, we aim to clarify the effects of DP on iron metabolism and the underlying mechanism. In this study, we found that DP could reduce iron burden in hepatoma cells and grafted tumors. Hepcidin is a central regulator in iron metabolism. We confirmed that the expression of hepcidin in HCC tumor tissues was significantly higher than that in the adjacent nontumor tissues. The expression of hepcidin was downregulated in the liver of mouse model treatment with DP, as well as in hepatoma cells. Moreover, RNA sequencing and western blot data revealed that DP inhibited the IL-6-activated JAK-STAT signaling pathway. In summary, our results revealed that DP might be a new potential drug candidate for the regulation of iron burden and the treatment of HCC.
最近的研究声称铁过载与肝细胞癌（HCC）的风险相关，我们之前的研究也表明蒲公英多糖（DP）通过引起细胞周期停滞和抑制 PI3K/AKT/mTOR 通路来抑制 HCC 细胞系的增殖，但 DP 对代谢的影响尚不清楚。在这里，我们旨在阐明 DP 对铁代谢的影响及其潜在机制。在这项研究中，我们发现 DP 可以降低肝癌细胞和移植瘤中的铁负荷。铁调素是铁代谢的中心调节剂。我们证实，HCC 肿瘤组织中的铁调素表达明显高于相邻非肿瘤组织。DP 处理的小鼠模型肝脏以及肝癌细胞中的铁调素表达下调。此外，RNA 测序和 Western blot 数据显示 DP 抑制了 IL-6 激活的 JAK-STAT 信号通路。总之，我们的结果表明 DP 可能是一种新的潜在药物候选物，可用于调节铁负荷和治疗 HCC。
