Kong Delong, Li Xiangyang, Zhang Beibei, Yan Chao, Tang Renxian, Zheng Kuiyang
Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, 221004, Jiangsu Province, People's Republic of China.
Iran J Basic Med Sci. 2020 Dec;23(12):1538-1543. doi: 10.22038/ijbms.2020.39436.9350.
Immune responses are tightly regulated in the development of clonorchiasis. However, the adaptive immune regulatory pathway contributing to the pathological processes of infection is still not clear. In this study, we assessed the dynamic changes of CD4T cell subsets and the related cytokines as well as transcription factors during infection.
We used female FVB mice to establish the infection model. H&E and Masson's stain were performed in 2 or 8 weeks post-infection (PI) liver of . The percentages of splenic Th1, Th2, and Treg in CD4+T cells were detected by flow cytometry. The transcription factors T-bet, GATA3, Foxp3, and RORγt gene expression were detected by qPCR. The protein expression of IL-10, IL-17, IL-4, IL-2, and Tumor Necrosis Factor-α (TNF-α) were examined using ELISA.
The percentages of splenic Th1, Th2, and Treg in CD4T cells were significantly increased in both 2 and 8 weeks PI of , while the ratio of Treg/Th17 as well as the percentage of Treg in serum was gradually increased during the development of infection. The expressions of T-bet, GATA3, Foxp3, and RORγt were increased in 8 weeks PI. Serum levels of IL-10, IL-17, IL-4, and IL-2 were profoundly increased in infected mice, while the concentrations of TNF-α increased to peak two weeks PI.
Our results suggested that the imbalance of CD4T cell subsets may regulate and contribute to an appropriate compromise between pathology, tissue repair, and elimination in a susceptible murine host.
华支睾吸虫病发展过程中免疫反应受到严格调控。然而，导致感染病理过程的适应性免疫调节途径仍不清楚。在本研究中，我们评估了感染过程中CD4T细胞亚群、相关细胞因子以及转录因子的动态变化。
我们使用雌性FVB小鼠建立感染模型。对感染后2周或8周的肝脏进行苏木精-伊红（H&E）染色和Masson染色。通过流式细胞术检测脾细胞中CD4+T细胞里Th1、Th2和调节性T细胞（Treg）的百分比。通过定量聚合酶链反应（qPCR）检测转录因子T-bet、GATA3、Foxp3和维甲酸相关孤儿受体γt（RORγt）基因的表达。使用酶联免疫吸附测定（ELISA）检测白细胞介素-10（IL-10）、白细胞介素-17（IL-17）、白细胞介素-4（IL-4）、白细胞介素-2和肿瘤坏死因子-α（TNF-α）的蛋白表达。
在感染后2周和8周，脾细胞中CD4T细胞里Th1、Th2和Treg的百分比均显著增加，而在感染发展过程中，Treg/Th17的比例以及血清中Treg的百分比逐渐升高。感染后8周，T-bet、GATA3、Foxp3和RORγt的表达增加。感染小鼠血清中IL-10、IL-17、IL-4和IL-2的水平显著升高，而TNF-α的浓度在感染后两周升至峰值。
我们的结果表明，CD4T细胞亚群的失衡可能在易感染的小鼠宿主中调节并促成病理、组织修复和清除之间的适当平衡。
