Kalantar-Neyestanaki Davood, Mansouri Shahla, Kandehkar Ghahraman Mohammad Reza, Tabatabaeifar Fatemehalsadat, Hashemizadeh Zahra
Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Basic Med Sci. 2020 Dec;23(12):1551-1557. doi: 10.22038/ijbms.2020.47520.10940.
is one of the most important causes of urinary tract infections (UTIs). The aim of this study was to determine antimicrobial resistance, resistance and virulence genes; phylogenetic groups and identify the epidemiologic features of uropathogenic (UPEC) isolates by multilocus sequence typing (MLST).
One hundred isolates of from inpatients with UTIs were collected in Kerman, Iran. Antimicrobial susceptibility testing, ESBLs, AmpC production and biofilm formation were performed by phenotypic methods. Phylogenetic groups, resistance and virulence genes were detected. Molecular typing of isolates was performed by MLST.
In this study, 76% of isolates were multidrug-resistant. The and were the dominant ESBL-encoding gene. Among 63 ciprofloxacin-resistant isolates, the frequency of (15.8%), (9.5%), and (6')- (25% ) genes was shown. Fifty-five present of isolates were classified as week biofilm, (14%) moderate biofilm, and (5%) strong. The predominant phylogenetic group was B2 (3) . The prevalence of virulence genes ranged (93%), (66%), (59%), (39%), (28%) and (27%). According to MLST results, 14 sequence types (ST) including ST-693, ST-90, ST-101, ST-1664, ST-2083, ST-131, ST-4443, ST-744, ST-361, ST-405, ST-922, ST-648, ST-5717and ST-410 were detected, indicating a high degree of genotypic diversity.
We identified a high frequency of the ST131 clonal group among UTIs. These data show an important public health threat, and so further studies to control the dissemination and risk factors for acquisition of the ST131 clonal group and other STs are needed to make effective control.
是尿路感染（UTIs）最重要的病因之一。本研究的目的是确定抗菌药物耐药性、耐药基因和毒力基因；系统发育群，并通过多位点序列分型（MLST）鉴定尿路致病性大肠埃希菌（UPEC）分离株的流行病学特征。
从伊朗克尔曼的住院UTIs患者中收集了100株大肠埃希菌分离株。采用表型方法进行抗菌药物敏感性试验、ESBLs检测、AmpC产生及生物膜形成检测。检测系统发育群、耐药基因和毒力基因。通过MLST对分离株进行分子分型。
在本研究中，76%的分离株对多种药物耐药。blaCTX-M和blaSHV是主要的ESBL编码基因。在63株对环丙沙星耐药的分离株中，检出qnrA（15.8%）、qnrB（9.5%）和qnrS（6′）-Ib（25%）基因的频率。55%的分离株被分类为弱生物膜，14%为中度生物膜，5%为强生物膜。主要的系统发育群是B2（35%）。毒力基因的流行率范围为 fimH（93%）、papC（66%）、sfa/foc（59%）、afa/draBC（39%）、hlyA（28%）和ibeA（27%）。根据MLST结果，检测到14种序列类型（ST），包括ST-69
