Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Ann Clin Microbiol Antimicrob. 2021 Sep 8;20(1):65. doi: 10.1186/s12941-021-00470-7.
This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing bla gene.
To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing bla gene.
According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B, followed by group D (18.9%), and group A (13.5%). Furthermore, except for phylogenetic group C, there was no significant correlation between phylogenetic distribution in KTPs and non-KTPs. Additionally, MLST analysis of bla carrying isolates identified 18 unique sequence types (ST) the most common of which was ST131 (24.1%), followed by ST1193 (10.3%), while fourteen STs were detected only once.
The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.
本研究旨在调查从肾移植患者（KTP）和非 KTP 中分离的尿路致病性大肠杆菌（UPEC）的系统发育特征和毒力特征，并分析携带 bla 基因的产Extended spectrum β-lactamases（ESBLs）的 UPEC 的克隆分布。
为此，我们确定了非 KTP（n=65）和 KTP（n=46）中 UPEC 的毒力标记物和系统发育特征。非 KTP 被认为是本研究的对照组。此外，根据 Achtman 方案，我们进行多位点序列分型以评估包含 bla 基因的 29 个 ESBL 产生分离株之间的关系。
根据 PCR 检测结果，毒力因子基因的流行率范围从 0%（cnf 和 papG III）到 93.7%（fimH）。此外，KTP 分离株与非 KTP 分离株仅在 pap GI 元件的流行率方面存在显著差异。此外，最常见的 UPEC 分离株属于 B 群，其次是 D 群（18.9%）和 A 群（13.5%）。此外，除了 C 群之外，KTP 和非 KTP 之间的系统发育分布之间没有显著相关性。此外，携带 bla 的分离株的 MLST 分析确定了 18 个独特的序列型，最常见的是 ST131（24.1%），其次是 ST1193（10.3%），而 14 个 ST 仅检测到一次。
结果进一步表明，KTP 和非 KTP 中 UPEC 分离株在 C 群和 PAI 基因方面存在显著差异。基于 MLST 分析，我们还观察到从 KTP 和非 KTP 中获得的 UPEC 分离株具有相对较高的多样性。此外，克隆复合体（CC）131 和 ST131 分别是最常见的克隆和 ST 类型。此外，首次在 KTP 中报告了 ST8503。这些结果表明应定期对 KTP 中的 UPEC 分离株进行特征研究。
