Pal Asmita, Verma Preeti, Paul Subhabrata, Majumder Indira, Kundu Rita
Department of Botany, Centre of Advanced Studies, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019 West Bengal India.
Present Address: Bioprospecting Laboratory, School of Biotechnology, Presidency University, Kolkata, West Bengal India.
3 Biotech. 2021 Feb;11(2):52. doi: 10.1007/s13205-020-02576-9. Epub 2021 Jan 11.
Edible green algal seaweeds, namely and constitute a significant repository of popular herbal medicines in the Traditional Chinese Medicinal system. The present study aimed to assess the anticancer potential of these algal members and its mode of action in cervical cancer cells SiHa. The algal samples primarily extracted in methanol was fractionated into hexane, chloroform, and aqueous algal fractions, which inhibited the proliferation of SiHa cells in a dose-dependent manner, with the algal chloroform fractions harbouring the lowest IC dose of 141.38 µg/ml in and 445.278 µg/ml in . These algal chloroform fractions when studied for their in-depth mode of action, were found to damage and pulverise the nuclei, resulting in a concomitant increase in subG0-phase of SiHa cells, studied by flow cytometry. The algal treatment also caused an increase in the number of acidic vesicles and enhanced the expression of LC3BII, p62 and atg12 proteins, which together pointed out autophagy as the induced mode of cell death. Upregulated Bax and p53 expression along with decreased Bcl2 expression also correlated to autophagic cell death. Decreased expression of E6 viral oncogene was noted as a significant response to algal fractions. Lastly, these potent algal fractions when characterised pharmacologically through GC-MS analysis were found to be rich in unsaturated fatty acids, majorly palmitic acid. Hence, this study concludes that the two species of successfully decreased the proliferation of SiHa cervical cancer cells through autophagy, hinting at palmitic acid being the major responsible bioactive compound in both.
可食用绿藻海藻，即[具体名称1]和[具体名称2]，是传统中药体系中重要的常用草药库。本研究旨在评估这些藻类成员的抗癌潜力及其在宫颈癌细胞SiHa中的作用模式。主要用甲醇提取的藻类样品被分离成己烷、氯仿和水相藻类馏分，它们以剂量依赖性方式抑制SiHa细胞的增殖，其中藻类氯仿馏分的最低半数抑制浓度（IC）在[具体名称1]中为141.38μg/ml，在[具体名称2]中为445.278μg/ml。当对这些藻类氯仿馏分的深入作用模式进行研究时，发现它们会破坏并粉碎细胞核，通过流式细胞术研究发现这会导致SiHa细胞的亚G0期随之增加。藻类处理还导致酸性囊泡数量增加，并增强了LC3BII、p62和atg12蛋白的表达，这些共同表明自噬是诱导的细胞死亡模式。Bax和p53表达上调以及Bcl2表达下降也与自噬性细胞死亡相关。E6病毒癌基因表达下降被认为是对藻类馏分的显著反应。最后，通过气相色谱 - 质谱（GC - MS）分析对这些有效的藻类馏分进行药理学表征时，发现它们富含不饱和脂肪酸，主要是棕榈酸。因此，本研究得出结论，这两种[藻类名称]通过自噬成功降低了SiHa宫颈癌细胞的增殖，表明棕榈酸是两者中主要的生物活性化合物。
