Chaguza Chrispin, Senghore Madikay, Bojang Ebrima, Lo Stephanie W, Ebruke Chinelo, Gladstone Rebecca A, Tientcheu Peggy-Estelle, Bancroft Rowan E, Worwui Archibald, Foster-Nyarko Ebenezer, Ceesay Fatima, Okoi Catherine, McGee Lesley, Klugman Keith P, Breiman Robert F, Barer Michael R, Adegbola Richard A, Antonio Martin, Bentley Stephen D, Kwambana-Adams Brenda A
Parasites and Microbes Programme, Wellcome Sanger Institute, Cambridge, United Kingdom.
Darwin College, University of Cambridge, Cambridge, United Kingdom.
Front Pediatr. 2021 Jan 8;8:587730. doi: 10.3389/fped.2020.587730. eCollection 2020.
(the pneumococcus) carriage precedes invasive disease and influences population-wide strain dynamics, but limited data exist on temporal carriage patterns of serotypes due to the prohibitive costs of longitudinal studies. Here, we report carriage prevalence, clearance and acquisition rates of pneumococcal serotypes sampled from newborn infants bi-weekly from weeks 1 to 27, and then bi-monthly from weeks 35 to 52 in the Gambia. We used sweep latex agglutination and whole genome sequencing to serotype the isolates. We show rapid pneumococcal acquisition with nearly 31% of the infants colonized by the end of first week after birth and quickly exceeding 95% after 2 months. Co-colonization with multiple serotypes was consistently observed in over 40% of the infants at each sampling point during the first year of life. Overall, the mean acquisition time and carriage duration regardless of serotype was 38 and 24 days, respectively, but varied considerably between serotypes comparable to observations from other regions. Our data will inform disease prevention and control measures including providing baseline data for parameterising infectious disease mathematical models including those assessing the impact of clinical interventions such as pneumococcal conjugate vaccines.
肺炎球菌携带先于侵袭性疾病并影响全人群的菌株动态，但由于纵向研究成本过高，关于血清型的时间性携带模式的数据有限。在此，我们报告了在冈比亚从出生第1周到第27周每两周、然后从第35周到第52周每两个月对新生儿进行采样所获得的肺炎球菌血清型的携带率、清除率和获得率。我们使用玻片乳胶凝集试验和全基因组测序对分离株进行血清分型。我们发现肺炎球菌获得迅速，近31%的婴儿在出生后第一周结束时被定植，2个月后迅速超过95%。在生命的第一年，每个采样点超过40%的婴儿持续被观察到携带多种血清型。总体而言，无论血清型如何，平均获得时间和携带持续时间分别为38天和24天，但不同血清型之间差异很大，这与其他地区的观察结果类似。我们的数据将为疾病预防和控制措施提供信息，包括为参数化传染病数学模型提供基线数据，这些模型包括评估肺炎球菌结合疫苗等临床干预措施影响的模型。
